Francesca Cella Zanacchi is currently a researcher at the IItalian Institute of Technology in Italy in the Nanophysics department. Previously, she worked as a Postdoc at the Institute de Ciències Fotòniques (ICFO) in Spain and as post-doctoral fellow in the Nanoscopy group at the Italian Institute of Technology. She graduated in Physics at the University of Genoa (Italy) in 2006 and in 2010 she accomplished her PhD studies in Physics (University of Genoa). She was a visitor scientist at EMBL (Heidelberg) in 2008 and at University of Maine (USA) in 2009. In December 2013 she joined the Single Molecule Biophysics group at University of Frankfurt as a short term EMBO fellowship. She works in the bioengineering/biophysics field and her research activity is focused on the design, realization and utilization of optical imaging techniques to study biomolecules at the molecular level in thick biological samples and tissues. Her work is mainly addressed to the study of biological molecules to investigate cell functioning using super-resolution microscopy, single molecule localization techniques (PALM, GSDIM), single molecule tracking, single plane illumination microscopy (SPIM), confocal, multiphoton fluorescence microscopy, nanostructured model systems and advanced fluorescence microscopy techniques (FRAP). She worked as a working package leader for the RENVISION European project (FP7-ICT-2011-9 (NBIS) # 600847) and she is active as member of several scientific societies and as reviewer for several international scientific journals.
October 2016-current Researcher at Nanophysics Dept. of the Istituto Italiano di Tecnologia (IIT).
May 2015-October 2016: Post Doc at the Institute of Photonics Sciences, Barcelona, Spain. Tutor Melike Lakadamyali, Project:"DNA origami as versatile calibration standard for quantitative single molecules localization techniques".
July 2014- May2015 Researcher at Nanophysics Dept. of the Istituto Italiano di Tecnologia (IIT).
Dec 2013-Feb 2014 EMBO Short term fellowship at University of Frankfurt, Germany. Tutor: Mike Heilemann. Project: "Advanced super-resolution and 3D tracking techniques for transcription studies in bacteria".
2010-July 2014 Post Doc at Nanophysics Dept (Nanoscopy group) of the Istituto Italiano di Tecnologia (IIT),Genova, Italy. Tutor: A Diaspro. Project: "Advanced light sheet based fluorescence microscopy techniques".
Aug 2009 –Dec 2009 Visiting research associate at University of Maine, Orono, Maine Tutor: S. Hess. "Development of a fluorescence single molecule localization localization microscope (FPALM) based on inclined illumination"
Aug 2008 –Dec 2008 Visiting scientist at "Light Microscopy Group"- EMBL Heidelberg, Germany Tutor: E.H.K. Stelzer. "Selective plane microscopy in scattering samples"
2007-2009 Ph.D. Degree in Physics, Department of Physics, University of Genoa, Italy
2006 MSc. Degree in Physics, Department of Physics, University of Genoa, Italy
International/national Prizes and Awards:
2013 International travel award - Biophysical society meeting 2013 (Phyladelphia)
2012 TR35-Giovani Innovatori Ed.2012
2012 NEST Prize per la Nanoscienza Ed. 2012
2011 Best poster award at BIOS 2011 SPIE Photonics West (SF)
2006 Prize for Degree Thesis in Physics: Premio di Studio GIC (Società Italiana Citometria) Menzione Speciale per la tesi di Laurea intitolata “Ruolo dei profili di eccitazione nello studio della diffusione di molecole fluorescenti” .
Activity within European projects:
Working Package leader within the EU project RENVISION (Retina-inspired ENcoding for advanced VISION tasks) awarded within the initiative of Future Emerging Technologies: Neuro-Bio-Inspired Systems (NBIS).
Reference no: EP17382394.9 "Method for quantifying protein copy number"
Main research projects:
F. Cella Zanacchi*, C. Manzo, A. Sandoval Alvarez, N. D. Derr, M. Garcia Parajo, M. Lakadamyali “A DNA Origami platform for quantifying protein copy-number in super-resolution“ Nature Methods 10.1038/nmeth.4342 (2017)
Cella Zanacchi F, Lavagnino Z., Perrone Donnorso M., Del Bue A., Furia L., Faretta M., Diaspro A. "Live-cell 3D superresolution imaging in thick biological samples" Nature Methods. 8 (12): DOI:10.1038/nmeth.1744 (2011).
Francesca Pennacchietti, Sebastiano Vascon, Thierry Nieus, Christian Rosillo, Sabyasachi Das, Shiva Tyagarajan, Alberto Diaspro, Alessio del Bue, Enrica Maria Petrini, Andrea Barberis and Francesca Cella Zanacchi* "Nanoscale molecular reorganization of the inhibitory postsynaptic density is a determinant of GABAergic synaptic potentiation" Journal of Neuroscience, 0514-16; DOI: https://doi.org/10.1523/JNEUROSCI.0514-16.2016 (2017)
Z. Lavagnino, G. Sancataldo, M.d’Amora, P. Follert, D. De Pietri Tonelli, A. Diaspro, F. Cella Zanacchi*, “4D (x-y-z-t) imaging of thick biological samples by means of Two Photon inverted Selective Plane Illumination Microscopy (2PE-iSPIM)” Sci Reports 6, (2016)
G.Hilgen, M. Sorbaro, S. Pirmoradian, J. Muthmann, I. Edit Kepiro, S. Ullo, C. Juarez Ramirez, A.Puente Encinas, Al. Maccione, L. Berdondini, V. Murino, D. Sona, F. Cella Zanacchi, E. Sernagor, M. H.Hennig "Unsupervised spike sorting for large-scale, high-density multielectrode arrays", Cell reports, 18 (10), (2017)
Lorén N., Hagman J., Jonasson J.K., Deschout H., Bernin D., Cella Zanacchi F., Diaspro A, McNally J.G., Ameloot M., Smisdom N., Nydén M., Hermansson H.M., Rudemo M. and Braeckmans K., “Fluorescence recovery after photobleaching in material and life sciences: putting theory into practice” Quarterly Reviews of Biophysics 48 (3), (2015)
Cella Zanacchi F.* Lavagnino Z., Faretta M., Furia L., Diaspro A., "Light-sheet confined super-resolution using two-photon photo-activation", Plos One 8 (7), oii:10.1371/journal.pone.0067667, (2013).
A. Pierzyńska-Mach, A Szczurek, F. Cella Zanacchi, F. Pennacchietti, J. Drukała, A.Diaspro, C. Cremer, Z. Darzynkiewicz, J. W Dobrucki "Subnuclear localization, rates and effectiveness of UVC-induced unscheduled DNA synthesis visualized by fluorescence widefield, confocal and super-resolution microscopy" Cell Cycle 15 (8) ,(2016).
Spahn C., Cella Zanacchi F., Ulrike Endesfelder U. and Heilemann M., “Correlative super-resolution imaging of RNA polymerase distribution and dynamics, bacterial membrane and chromosomal structure in Escherichia coli”, Methods and Applications in Fluorescence, Volume 3 (1), (2015)
Lavagnino Z., Cella Zanacchi F.*, Ronzitti E., and Diaspro A. Two-photon excitation selective plane illumination microscopy (2PE-SPIM) of highly scattering samples: characterization and application Optics Express, Vol. 21, Issue 5, pp. 5998-6008 (2013)
Chacko, J. V., Cella Zanacchi F. and Diaspro, A., "Probing cytoskeletal structures by coupling optical superresolution and AFM techniques for a correlative approach" Cytoskeleton 7(11), doi: 10.1002/cm.21139 (2013)
M. Perrone Donnorso, E. Miele, F. De Angelis, R. La Rocca, T. Limongi, F. Cella Zanacchi, S. Marras, R. Brescia and E. Di Fabrizio. Nanoporous Silicon Nanoparticles for drug delivery applications. Microelectronic Engineering. 2012.
Mazza D., Braeckmans K., Cella F., Testa I., Vercauteren D., Demeester J., DeSmedt S.S., Diaspro A. . A new FRAP/FRAPa method for three-dimensional diffusion measurements based on multiphoton excitation microscopy. Biophys Journal 95(7), 3457–69 (2008)
Cella Zanacchi F.Proc. of SPIE, vol.7903 “Multiphoton Microscopy in the Biomedical Sciences XI” "Two-photon fluorescence excitation within a light sheet based microscopy architecture" (2011)
Cella F., Lavagnino Z., Diaspro A, Non-linear effects and role of scattering in multiphoton imaging of thick biological samples. Multiphoton Microscopy in the Biomedical Sciences IX Proceedings of the SPIE, Volume 7183, pp. 718324-718324-7 (2009)
Cella F., Ronzitti E, Vicidomini G, Diaspro A, Studying the illumination puzzle towards an isotropic increase of optical Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XV (J.Conchello, C.J.Cogswell, T.Wilson, T.G.Brown) Proc. of SPIE Vol. 6861, 686112, (2008)