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Paolo Bianchini

Nanoscopy & NIC@IIT
Research center

His main research activity aims at the biophysical study of biological molecules and macromolecules both in situ and in vitro utilizing almost not perturbative instrumentation and nanostructured model systems. So far, his research deals with the design, realization and utilization of biophysical instrumentation as conventional, confocal and multi-photon fluorescence microscopy, SHG imaging microscopy, FLIM, single molecule imaging, STED nanoscopy. In particular, he developed an original setup for SW 2PE-STED (single wavelength two-photon excitation stimulated emission depletion) super-resolution microscopy and another for SHG (second harmonic generation) combined with 2PEF (2-photon excitation fluorescence) microscopy. He recently applied STED nanoscopy technique to the quantitative study of single molecule diffusion in living cells. Moreover, he is in charge of the scientific management of the Nikon Imaging Center and he is responsible for the partnership with the R&D of Nikon Instruments Japan.

Top Publications
Storti B., Quaranta P., Di Primio C., Clementi N., Mancini N., Criscuolo E., Spezia P.G., Carnicelli V., Lottini G., Paolini E., Freer G., Lai M., Costa M., Beltram F., Diaspro A., Pistello M., Zucchi R., Bianchini P., Signore G., Bizzarri R.
A spatial multi-scale fluorescence microscopy toolbox discloses entry checkpoints of SARS-CoV-2 variants in Vero E6 cells
Computational and Structural Biotechnology Journal, vol. 19, pp. 6140-6156
Jadavi S., Bianchini P., Cavalleri O., Dante S., Canale C., Diaspro A.
Correlative nanoscopy: A multimodal approach to molecular resolution
Microscopy Research and Technique, vol. 84, (no. 10), pp. 2472-2482
Bianchini P., Saggau P., Saggau P., Diaspro A., Diaspro A.
An inertia-free beam scanning device for single-wavelength 2PE-STED nanoscopy
Journal of Physics D: Applied Physics, vol. 53, (no. 32)
Deguchi T., Bianchini P., Palazzolo G., Oneto M., Diaspro A., Duocastella M.
Volumetric Lissajous confocal microscopy with tunable spatiotemporal resolution
Biomedical Optics Express, vol. 11, (no. 11), pp. 6293-6310
Cosentino M., Canale C., Bianchini P., Diaspro A.
AFM-STED correlative nanoscopy reveals a dark side in fluorescence microscopy imaging
Science advances, vol. 5, (no. 6)
All Publications
Sheppard C.J.R., Castello M., Tortarolo G., Zunino A., Slenders E., Bianchini P., Vicidomini G., Diaspro A.
Signal strength and integrated intensity in confocal and image scanning microscopy
Journal of the Optical Society of America A, vol. 40, pp. 138-148
Jadavi S., Dante S., Bianchini P., Diaspro A., Canale C.
AFM-STED correlative nanoscopy applied to the study of in-vitro misfolded protein aggregation
Biophysical Journal, vol. 121, (no. 3), pp. 279a
Article Journal
Storti B., Carlotti B., Chiellini G., Ruglioni M., Salvadori T., Scotto M., Elisei F., Diaspro A., Bianchini P., Bizzarri R.
An Efficient Aequorea victoria Green Fluorescent Protein for Stimulated Emission Depletion Super-Resolution Microscopy
International Journal of Molecular Sciences, vol. 23, (no. 5)
Bruno S., Margiotta M., Cozzolino M., Bianchini P., Diaspro A., Cavanna L., Tognolini M., Abbruzzetti S., Viappiani C.
A photosensitizing fusion protein with targeting capabilities
Biomolecular Concepts, vol. 13, (no. 1), pp. 175-182
Garzella F., Bianchini P., Diaspro A., Losi A., Gartner W., Abbruzzetti S., Viappiani C.
A red-green photochromic bacterial protein as a new contrast agent for improved photoacoustic imaging
Photoacoustics, vol. 26
Awards and Achievements
Bianchini P.
TR35-Young Innovators Italia
Bianchini P.
Frontiers in Physics
Bianchini P.
European Biophysics Journal
Bianchini P.
Microscopy Research and Technique
Bianchini P.
Frontiers in Bioengineering and Biotechnology, Nanobiotechnology
Organized Events
Diaspro A., Bianchini P.
7th NIC@IIT Advanced Microscopy practical workshop
Diaspro A., Sheppard C.J.R., Bianchini P.
Diaspro A., Bianchini P.
6th NIC@IIT advanced practical workshop
Diaspro A., Bianchini P., Giacometti G.
IVSLA International School on Nanoscale Optical Microscopy
Diaspro A., Bianchini P.
5th NIC@IIT advanced practical workshop