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Nunzio Del Gaudio

Post Doc

Research Line

Genomic Science

Center

CGS@SEMM Milano

Contacts

About

 

Education

31/10/2016 PhD in Biochemistry and Biotechnology, University of Campania “L. Vanvitelli” (Italy)

26/03/2013 Master's degree in Medical Biotechnology, University of Naples “Federico II” (Italy)

17/12/2010 Bachelor degree in Biotechnology for Health, University of Naples “Federico II” (Italy)

Research experiences

04/03/2017 – present Postdoc at Italian Institute of Technology- Center for Genomic Science

Research activity: Epitranscriptome regulation of RNA dynamics and their alteration in cancer 

15/05/2017-28/02/2018 Postdoc at University of Campania “L.Vanvitelli”, Napoli (Italy)

Research activity: Identification and characterization of novel epigenetic targets with a role in the establishment and/or maintenance of human leukemia

01/11/2013-31/10/2016 PhD student at University of Campania “L.Vanvitelli”, Napoli (Italy)

Research activity: Identification and validation of novel epigenetic targets involved in the human acute myeloid leukemia

01/04/2016–31/07/2016 Visiting PhD student at RadBoud University, Nijmegen (Netherlands)

Research activity: Identification of new chromatin target interactors by mass spectrometry-based interaction proteomics

01/03/2012–31/08/2012 Master's degree internship at Univeristy of Amsterdam, Amsterdam (Netherlands)

Research activity: Role of ANF enhancers in regulating ANF expression in embryonic mouse heart

Technical skills and competences

Molecular cloning. Preparation, extraction, purification and analysis of DNA and/or mRNA from eukaryotic cells. PCR technology: amplification of DNA by PCR, PCR mutagenesis, RT-PCR, real-time PCR. Cell culture of primary and transformed mammalian cells. Stable and transient cell transfection, retrovirus and lentivirus production and transduction. Proliferation assay (soft agar, clonogenic assay), apoptosis assays (annexin V and tunel assays), caspase activity assay, cell viability assay (MTT), reporter assays (luciferase and galactosidase). In vitro transcription and translation assay. Protein expression analysis using common electrophoresis techniques, Western blot. Protein interaction technologies: GST, GFP, MBP pulldown, co-immunoprecipitation assay. Post-translational modification assays such as λ-phosphatase assay (λ-PPase), ubiquitination assay, SUMOylation assay. Cell cycle profiles, apoptosis and differentiation by FACS analysis. Analysis of protein-DNA interaction by chromatin immunoprecipitation, analysis of protein-RNA interaction by RNA immunoprecipitation. Microarray and RNA-Seq. ChIP-seq .Immunofluorescence assay. Mass spectrometry-based interaction proteomics.

Awards

17-12-2015 Recipient of a “Blueprint Epigenome Exchange Program” travel grant

Conferences

COST: Chromatin, Epigenome and Drug Discovery Training School (Napoli IT, 2016)

The 4th BLUEPRINT Consortium Meeting (Cambridge UK, 2015)

4th Oxford Symposium - New Frontiers in Epigenetics (Oxford UK, 2015)

HDAC inhibitors - Lessons learned and challenges for the future (Napoli IT, 2015)

The 3rd BLUEPRINT Consortium Meeting (Roma IT, 2014)

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I numeri di IIT

L’Istituto Italiano di Tecnologia (IIT) è una fondazione di diritto privato - cfr. determinazione Corte dei Conti 23/2015 “IIT è una fondazione da inquadrare fra gli organismi di diritto pubblico con la scelta di un modello di organizzazione di diritto privato per rispondere all’esigenza di assicurare procedure più snelle nella selezione non solo nell’ambito nazionale dei collaboratori, scienziati e ricercatori ”.

IIT è sotto la vigilanza del Ministero dell'Istruzione, dell'Università e della Ricerca e del Ministero dell'Economia e delle Finanze ed è stato istituito con la Legge 326/2003. La Fondazione ha l'obiettivo di promuovere l'eccellenza nella ricerca di base e in quella applicata e di favorire lo sviluppo del sistema economico nazionale. La costruzione dei laboratori iniziata nel 2006 si è conclusa nel 2009.

Lo staff complessivo di IIT conta circa 1440 persone. L’area scientifica è rappresentata da circa l’85% del personale. Il 45% dei ricercatori proviene dall’estero: di questi, il 29% è costituito da stranieri provenienti da oltre 50 Paesi e il 16% da italiani rientrati. Oggi il personale scientifico è composto da circa 60 principal investigators, circa 110 ricercatori e tecnologi di staff, circa 350 post doc, circa 500 studenti di dottorato e borsisti, circa 130 tecnici. Oltre 330 posti su 1400 creati su fondi esterni. Età media 34 anni. 41% donne / 59 % uomini.

Nel 2015 IIT ha ricevuto finanziamenti pubblici per circa 96 milioni di euro (80% del budget), conseguendo fondi esterni per 22 milioni di euro (20% budget) provenienti da 18 progetti europei17 finanziamenti da istituzioni nazionali e internazionali, circa 60 progetti industriali

La produzione di IIT ad oggi vanta circa 6990 pubblicazioni, oltre 130 finanziamenti Europei e 11 ERC, più di 350 domande di brevetto attive, oltre 12 start up costituite e altrettante in fase di lancio. Dal 2009 l’attività scientifica è stata ulteriormente rafforzata con la creazione di dieci centri di ricerca nel territorio nazionale (a Torino, Milano, Trento, Parma, Roma, Pisa, Napoli, Lecce, Ferrara) e internazionale (MIT ed Harvard negli USA) che, unitamente al Laboratorio Centrale di Genova, sviluppano i programmi di ricerca del piano scientifico 2015-2017.

IIT: the numbers

Istituto Italiano di Tecnologia (IIT) is a public research institute that adopts the organizational model of a private law foundation. IIT is overseen by Ministero dell'Istruzione, dell'Università e della Ricerca and Ministero dell'Economia e delle Finanze (the Italian Ministries of Education, Economy and Finance).  The Institute was set up according to Italian law 326/2003 with the objective of promoting excellence in basic and applied research andfostering Italy’s economic development. Construction of the Laboratories started in 2006 and finished in 2009.

IIT has an overall staff of about 1,440 people. The scientific staff covers about 85% of the total. Out of 45% of researchers coming from abroad 29% are foreigners coming from more than 50 countries and 16% are returned Italians. The scientific staff currently consists of approximately 60 Principal Investigators110 researchers and technologists350 post-docs and 500 PhD students and grant holders and 130 technicians. External funding has allowed the creation of more than 330 positions . The average age is 34 and the gender balance proportion  is 41% female against 59% male.

In 2015 IIT received 96 million euros in public funding (accounting for 80% of its budget) and obtained 22 million euros in external funding (accounting for 20% of its budget). External funding comes from 18 European Projects, other 17 national and international competitive projects and approximately 60 industrial projects.

So far IIT accounts for: about 6990 publications, more than 130 European grants and 11 ERC grants, more than 350 patents or patent applications12 up start-ups and as many  which are about to be launched. The Institute’s scientific activity has been further strengthened since 2009 with the establishment of 11 research nodes throughout Italy (Torino, Milano, Trento, Parma, Roma, Pisa, Napoli, Lecce, Ferrara) and abroad (MIT and Harvard University, USA), which, along with the Genoa-based Central Lab, implement the research programs included in the 2015-2017 Strategic Plan.