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Luca Lanzano

Researcher

Research Line

Nanoscopy & NIC@IIT

Center

IIT Central Research Labs Genova

Contacts

+39 010 2896 461
Contact Me

About

Past Employment

(2008-2013) Postdoc/Core Group Leader. Laboratory for Fluorescence Dynamics (LFD) – Department of Biomedical Engineering. University of California, Irvine, USA.
Main Research Projects:
-Develop and apply methods based on fluorescence microscopy (FRET, phasor analysis, Single Particle Tracking, FCS) for the study of protein-protein interaction and dynamics in live epithelial cell microvilli
-Development of a novel imaging method based on Particle Tracking for 3D imaging of subcellular structures with nanometer resolution
-Development of a method to measure dynamics of single molecules within single nuclear pore complexes in live cells
-Implementation of the Orbital Tracking method on a commercial microscope
Teaching Activity:
-Lecturer and teaching assistant of the course: Biospectroscopy (BME 295) during years 2010, 2011, 2012, 2013 – Department of Biomedical Engineering - University of California, Irvine
Advisor: Prof Enrico Gratton.


Education

(2008)  Ph.D. in Physics, cum laude. University of Catania, Italy.
Thesis title: “Delayed Luminescence as a tool to investigate the biological organization”.

(2007)  Visiting researcher at Laboratory for Fluorescence Dynamics, University of California, Irvine.
Major activities:
– Image correlation and 3D imaging methods to investigate cell dynamics during wound healing
– Number & Brightness analysis of protein aggregation in neurodegenerative disease
– Fluctuation analysis to detect stiffness of collagen fibers

(2004) M.S. Physics, summa cum laude. University of Catania, Italy.
Thesis title: “Study of the interaction between Si-nanoclusters and Er3+ ions by means of variable time length laser pulse excitation”

Projects

(1)   Develop of a novel super-resolution method based on the encoding of spatial information into fluorescence dynamics

(2)   Application of live-cell compatible super-resolution methods to the investigation of large scale organization in chromatin

(3)   Super-resolution imaging of replication and transcription in cellular models of cancer

Selected Publications

Sarmento M., M. Oneto, S. Pelicci, L. Pesce, L. Scipioni, M. Faretta, L. Furia, G.I. Dellino, P.G. Pelicci, P. Bianchini, A. Diaspro and L. Lanzanò. Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures. Nature Communications (2018) 9:3415 DOI:10.1038/s41467-018-05963-2

L. Scipioni, M. Di Bona, G. Vicidomini, A. Diaspro & L. Lanzanò. Local raster image correlation spectroscopy generates high-resolution intracellular diffusion maps. Communications Biology (2018) 1: (2018) 1:10 [https://www.nature.com/articles/s42003-017-0010-6]

L. Lanzanò, L. Scipioni, M. Di Bona, P. Bianchini, R. Bizzarri, F. Cardarelli, A. Diaspro & G. Vicidomini. Measurement of nanoscale three-dimensional diffusion in the interior of living cells by STED-FCS. Nature Communications 8:65 DOI: 10.1038/s41467-017-00117-2 (2017)

Scipioni L., E. Gratton, A. Diaspro, L. Lanzanò. Phasor Analysis of Local ICS Detects Heterogeneity in Size and Number of Intracellular Vesicles, Biophysical Journal (2016), 111, 619–629

L Lanzanò, I C Hernandez, M Castello, E Gratton, A Diaspro, and G Vicidomini. Encoding and decoding spatio-temporal information for super-resolution microscopy. Nature Communications 6:6701 (2015)

M D Brenner, R Zhou, D E. Conway, L Lanzanò, E Gratton, M A. Schwartz and T Ha.  Spider Silk Peptide Is a Compact, Linear Nanospring Ideal for Intracellular Tension Sensing. Nano Lett. (2016), 16 (3), 2096–2102

Lanzanò, L., and E. Gratton. Orbital single particle tracking on a commercial confocal microscope using piezoelectric stage feedback. Methods and Applications in Fluorescence 2.2 (2014): 024010.

L Lanzanò*, I Altamore* and E Gratton (2013) Dual channel detection of ultra low concentration of bacteria in real time by scanning fluorescence correlation spectroscopy. Meas. Sci. Technol. 24 065702  (*equally contributing authors)

H. Giral*, D.A. Cranston*, L. Lanzano*, Y. Caldas, E. Sutherland, J. Rachelson, E. Dobrinskikh, E. J. Weinman, R. B. Doctor, E. Gratton, and M. Levi (2012) NHE3 Regulatory Factor 1 (NHERF1) Modulates Intestinal Na-dependent Phosphate Transporter (NaPi-2b) Expression in Apical Microvilli. Journal of Biological Chemistry 287(42): 35047-35056 (*equally contributing authors)

F. Cardarelli, L. Lanzano, and E. Gratton (2012). Capturing directed molecular motion in the nuclear pore complex of live cells. Proceedings of the National Academy of Sciences, 109 (25): 9863-9868

L. Lanzano and E. Gratton (2012). Measurement of Distance With the Nanoscale Precise Imaging by Rapid Beam Oscillation Method. Microscopy Research and Technique 75(9): 1253-1264

H. Giral*, L. Lanzano*, Y. Caldas, J. Blaine, J. W. Verlander, T. Lei, E. Gratton, M. Levi (2011). Role of PDZK1 protein in apical membrane expression of renal sodium-coupled phosphate transporters. Journal of Biological Chemistry,  286; 15032-15042 (*equally contributing authors)

L. Lanzano*, T. Lei*, K. Okamura*, H. Giral, Y. A. Caldas, O. Masihzadeh, E. Gratton, M. Levi, J. Blaine (2011). Differential modulation of the molecular dynamics of the type IIa and IIc sodium phosphate cotransporters by parathyroid hormone. American Journal of Physiology. Cell Physiology 301(4): C850-861 (*equally contributing authors)

L. Lanzano, M. A. Digman, P. Fwu, H. Giral, M. Levi, and E. Gratton (2011). Nanometer-scale imaging by the modulation tracking method. Journal of Biophotonics, vol. 4; p. 415-424, doi: 10.1002/jbio.201100002

Patents

L. Lanzano, M. Digman and E. Gratton - Nanometer-Scale Optical Imaging by the Modulation Tracking (MT) method - US Patent 20120250000, 04 Oct 2012

Awards

Grants

 (2016) TRIDEO (TRansforming IDEas in Oncological research) award for the proposal: ‘Super-resolution imaging of the organization of transcription and replication during oncogene-induced replicative stress’ funded by AIRC (Italian Association for Cancer Research) (84.7k€ / 2 years)

Awards

(2016)  Winner of an AIRC-TRIDEO award

(2016) Winner of a Biophysical Society International Travel Award to attend the Biophysical Society 60th Annual Meeting, Los Angeles (CA)

(2015) Winner of a SIBPA Travel Award to attend the 59th Biophysical Meeting, Baltimore (MD)

(2013) Finalist for the ISSNAF Awards, Washington, DC October 29th, 2013

(2012) American Physiological Society Epithelial Transport Group Young Investigator Award

(1995) Winner of the 4th “Etniade di Matematica”, Department of Mathematics and Computer Sciences, University of Catania

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I numeri di IIT

L’Istituto Italiano di Tecnologia (IIT) è una fondazione di diritto privato - cfr. determinazione Corte dei Conti 23/2015 “IIT è una fondazione da inquadrare fra gli organismi di diritto pubblico con la scelta di un modello di organizzazione di diritto privato per rispondere all’esigenza di assicurare procedure più snelle nella selezione non solo nell’ambito nazionale dei collaboratori, scienziati e ricercatori ”.

IIT è sotto la vigilanza del Ministero dell'Istruzione, dell'Università e della Ricerca e del Ministero dell'Economia e delle Finanze ed è stato istituito con la Legge 326/2003. La Fondazione ha l'obiettivo di promuovere l'eccellenza nella ricerca di base e in quella applicata e di favorire lo sviluppo del sistema economico nazionale. La costruzione dei laboratori iniziata nel 2006 si è conclusa nel 2009.

Lo staff complessivo di IIT conta circa 1440 persone. L’area scientifica è rappresentata da circa l’85% del personale. Il 45% dei ricercatori proviene dall’estero: di questi, il 29% è costituito da stranieri provenienti da oltre 50 Paesi e il 16% da italiani rientrati. Oggi il personale scientifico è composto da circa 60 principal investigators, circa 110 ricercatori e tecnologi di staff, circa 350 post doc, circa 500 studenti di dottorato e borsisti, circa 130 tecnici. Oltre 330 posti su 1400 creati su fondi esterni. Età media 34 anni. 41% donne / 59 % uomini.

Nel 2015 IIT ha ricevuto finanziamenti pubblici per circa 96 milioni di euro (80% del budget), conseguendo fondi esterni per 22 milioni di euro (20% budget) provenienti da 18 progetti europei17 finanziamenti da istituzioni nazionali e internazionali, circa 60 progetti industriali

La produzione di IIT ad oggi vanta circa 6990 pubblicazioni, oltre 130 finanziamenti Europei e 11 ERC, più di 350 domande di brevetto attive, oltre 12 start up costituite e altrettante in fase di lancio. Dal 2009 l’attività scientifica è stata ulteriormente rafforzata con la creazione di dieci centri di ricerca nel territorio nazionale (a Torino, Milano, Trento, Parma, Roma, Pisa, Napoli, Lecce, Ferrara) e internazionale (MIT ed Harvard negli USA) che, unitamente al Laboratorio Centrale di Genova, sviluppano i programmi di ricerca del piano scientifico 2015-2017.

IIT: the numbers

Istituto Italiano di Tecnologia (IIT) is a public research institute that adopts the organizational model of a private law foundation. IIT is overseen by Ministero dell'Istruzione, dell'Università e della Ricerca and Ministero dell'Economia e delle Finanze (the Italian Ministries of Education, Economy and Finance).  The Institute was set up according to Italian law 326/2003 with the objective of promoting excellence in basic and applied research andfostering Italy’s economic development. Construction of the Laboratories started in 2006 and finished in 2009.

IIT has an overall staff of about 1,440 people. The scientific staff covers about 85% of the total. Out of 45% of researchers coming from abroad 29% are foreigners coming from more than 50 countries and 16% are returned Italians. The scientific staff currently consists of approximately 60 Principal Investigators110 researchers and technologists350 post-docs and 500 PhD students and grant holders and 130 technicians. External funding has allowed the creation of more than 330 positions . The average age is 34 and the gender balance proportion  is 41% female against 59% male.

In 2015 IIT received 96 million euros in public funding (accounting for 80% of its budget) and obtained 22 million euros in external funding (accounting for 20% of its budget). External funding comes from 18 European Projects, other 17 national and international competitive projects and approximately 60 industrial projects.

So far IIT accounts for: about 6990 publications, more than 130 European grants and 11 ERC grants, more than 350 patents or patent applications12 up start-ups and as many  which are about to be launched. The Institute’s scientific activity has been further strengthened since 2009 with the establishment of 11 research nodes throughout Italy (Torino, Milano, Trento, Parma, Roma, Pisa, Napoli, Lecce, Ferrara) and abroad (MIT and Harvard University, USA), which, along with the Genoa-based Central Lab, implement the research programs included in the 2015-2017 Strategic Plan.