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Paola Parlanti

Post Doc
Post Doc
Electron Crystallography
Address
Research center
About
Education

Title: PhD in Molecular Biophysics
Institute: Scuola Normale Superiore
Location: Pisa
Country: Italy
From: 2013 To: 2018

Title: Master Degree in Biology Applied to Biomedicine
Institute: Università di Pisa
Location: Pisa
Country: Italy
From: 2010 To: 2013

Title: Bachelor Degree in Molecular Biology
Institute: Università di Pisa
Location: Pisa
Country: Italy
From: 2005 To: 2010

Experience External

Title: Post Doc
Institute: Rutgers University
Location: New Brunswick, NJ
Country: USA
From: 2019 To: 2020

Title: Post Doc Research Associate
Institute: George Washington University
Location: Washington, DC
Country: USA
From: 2018 To: 2019

Title: Visiting
Institute: George Washington University
Location: Washington, DC
Country: USA
From: 2017 To: 2017

Title: PhD Student
Institute: Scuola Normale Superiore
Location: Pisa
Country: Italia
From: 2013 To: 2018

Skills

Electron Microscopy
- TEM
- FIB-SEM
- SEM
- Serial Block Face SEM
- EDX

Sample preparation for Electron Microscopy
- Lamella preparation (FIB-SEM)
- Embedding and ultramicrotome sectioning (bio samples)
- High pressure freezing and freeze substitution
- Critical point drying

Molecular Biology / Biochemistry / Neuroscience
- Cell culture, genetics, molecular biology and biochemistry techniques (PCR, Western blot...)
- Neuroscience: animal models, treatments and behavioral tests

Confocal microscopy
- ICC IF
- IHC IF

X-ray micro-CT (synchrotron)
- Sample prep for micro-CT (bio)

Correlative microscopy
- TEM/micro-CT
- FIB-SEM/micro-CT
- confocal/FIB-SEM

Image analysis
- Arivis
- MAPS
- Amira
- Imaris

All Publications
2021
Parlanti P., Pal-Ghosh S., Williams A., Tadvalkar G., Popratiloff A., Stepp M.A.
Axonal Debris Accumulates in Corneal Epithelial Cells After Intraepithelial Corneal Nerves are Damaged: a FIB-SEM Study
13th Annual FIB-SEM User Meeting
Conference Paper Conference
2021
Wagner C.L., Lippert P.C., Lascu I., Parlanti P., Polson R.
Searching for Magnetic Needles in Marine Haystacks: Promise and Progress with in situ Visualization of Magnetofossils in Marine Sediment Chips
13th Annual FIB-SEM User Meeting
Conference Paper Conference
2021
Suosaari E.P., Lascu I., Parlanti P., Oehlert A.M., Piggot A.M., Reid P.R.
Using FIB-SEM to Visualize the Submicron-Scale Association Between Microbes, Biogenic Mg-clay, and Carbonates
13th Annual FIB-SEM User Meeting
Conference Paper Conference
2020
DeDreu J., Bowen C.J., Logan C.M., Pal-Ghosh S., Parlanti P., Stepp M.A., Menko A.S.
An immune response to the avascular lens following wounding of the cornea involves ciliary zonule fibrils
FASEB Journal, vol. 34, (no. 7), pp. 9316-9336
2020
Parlanti P., Pal-Ghosh S., Williams A., Tadvalkar G., Popratiloff A., Stepp M.A.
Axonal debris accumulates in corneal epithelial cells after intraepithelial corneal nerves are damaged: A focused Ion Beam Scanning Electron Microscopy (FIB-SEM) study
Experimental Eye Research, vol. 194
Article Journal
Scientific Talks
2019
Parlanti P.
Epithelial cells – axons interaction in the wounded cornea: an extensive analysis through FIB-SEM
GWNIC Correlative Light and Electron Microscopy Workshop
Workshop/Symposium
2018
Parlanti P.
Correlative microscopy: overcoming single-method limits to address biological challenges
GWNIC Correlative Light and Electron Microscopy Workshop
Workshop/Symposium
2017
Parlanti P.
Correlative workflow from fluorescence to 3D ultrastructural reconstruction of intracellular organelles via FIB-SEM. Segmentation and volume reconstruction with Arivis
GWNIC Correlative Microscopy Workshop
Workshop/Symposium
Oral presentations
2021
Parlanti P., Pal-Ghosh S., Williams A., Tadvalkar G., Popratiloff A., Stepp M.A.
Axonal Debris Accumulates in Corneal Epithelial Cells After Intraepithelial Corneal Nerves are Damaged: a FIB-SEM Study
13th Annual FIB-SEM User Meeting
Conference
2019
Parlanti P., Oaks A.W., Brantner C.A., Popratiloff A., Manzini M.C.
Correlative workflow from fluorescence to 3D ultrastructural reconstruction of intracellular organelles via FIB-SEM imaging
12th Annual FIB-SEM User Meeting
Conference
2017
Rigolio R., Parlanti P., Cavaletti G., Gemmi M., Cappello V.
Size and Specimen-Dependent Strategy for X-Ray micro-CT and TEM Correlative Analysis of Nervous System Samples
NeuroMI
Conference