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Kiril Tuntevski

Visiting scientist

Research Line

Neurobiology of miRNA

Center

IIT Central Research Labs Genova

Contacts

About

PUBLICATIONS and PATENTS

  • Tuntevski K, Durney BC, Snyder AK, LaSala PR, Nayak AP, Green BJ, Beezhold DH, Rio RV, Holland LA, Lukomski S. 2013. Aspergillus Collagen-Like Genes (acl): Identification, Sequence Polymorphism, and Assessment for PCR-Based Pathogen Detection. Appl Environ Microbiol: 79(24): 7882-95;
  • Tuntevski K, Ellison R, Yakovenko S. 2016. Asymmetric Walkway: A Novel Behavioral Assay for Studying Asymmetric Locomotion. J Vis Exp:1–6 Available at: http://www.ncbi.nlm.nih.gov/pubmed/26863182;
  • Yakovenko S, Boots, M, Ellison R, & Tuntevski K. Walkway Device and Method for Quantitative Analysis of Gait and its Modification in Rodents. 9839200-B2 (2017).
  • Tuntevski K. 2018. Flexor Dysfunction Following Unilateral Transient Ischemic Brain Injury Is Associated with Impaired Locomotor Rhythmicity. (West Virginia University, 2018 – Thesis).

POSTER PRESENTATIONS

  • Tuntevski K, & Lukomski S. Distribution and Sequence Polymorphism in Collagen-Like Genes of the Pathogenic Fungus Aspergillus fumigatus. In Summer Research Symposium, Morgantown WV (West Virginia University, Morgantown WV, 2010); 
  • Tuntevski K, Rittenour W, Green B, Beezhold D, & Lukomski S. Distribution and Sequence Polymorphism in Collagen-Like Genes of the Pathogenic Fungus Aspergillus fumigatus. In STaR Symposium, Marshall University, Huntington WV (West Virginia Higher Education Policy Commission - Science, Technology and Research Symposium, 2010);  
  • Tuntevski K, & Lukomski S. Towards detection of invasive Aspergillosis. In Summer Research Symposium, Morgantown WV (West Virginia University, Morgantown WV, 2011);  
  • Tuntevski K, Rittenour WR, Tarlovsky Y, Green BJ, Beezhold DH, & Lukomski S. Assessment of CollagenLike Genes as Biomarkers for the Detection of the Pathogenic Fungus Aspergillus fumigatus. In 111th Symposium of the American Society for Microbiology (ASM, New Orleans LA, 2011); 
  • Tuntevski K, & Lukomski S. Assessment of Collagen-Like Genes as Biomarkers for the Detection of the Pathogenic Fungus Aspergillus fumigatus. In Undergraduate Research Symposium, University of Charleston WV (University of Charleston, Charleston WV, 2011); 
  • Bachert B., Tuntevski K. & Lukomski S. Assessment of Collagen-Like Genes, bucl and acl, as Targets for Detection of Select and Environmental Agents. In Bioelectronics and Biosensing Symposium (WV NanoSAFE, Morgantown WV, 2013); 
  • Tuntevski K, Shaffer JM, Ellison R, Pugacheva A & Yakovenko S. Asymmetric Walkway : Novel behavioral assay for studying asymmetric locomotion. In 49th Annual Van Lierie Research Symposium (West Virginia University School of Medicine, Morgantown WV, 2014); 
  • Tuntevski K, Ledford V, Shaffer JM & Yakovenko S. Induced asymmetric sensorimotor adaptations in rats mirror post-stroke gait. In Summer Research Symposium (West Virginia University School of Medicine, Morgantown WV, 2014); 
  • Tuntevski K, Ellison R, Shaffer JM, & Yakovenko S. Asymmetric Walkway: Novel behavioral assay for studying asymmetric locomotion. In Neuroscience 2014 (Society for Neuroscience, November 15-19, 2014, Washington DC). 
  • Tuntevski K, Freeman S, Yakovenko S. Towards a Minimalistic Central Pattern Generator for Quadrupedal Locomotion. In Neuroscience 2015 (Society for Neuroscience, October 17-21, 2015, Chicago, IL); 
  • Tuntevski K, Freeman S, Yakovenko S. 2015. Towards a Minimalistic Central Pattern Generator for Quadrupedal Locomotion. In Neuroscience Retreat (NeuroRetreat, WVU School of Medicine, July, 2015, Davis, WV); 
  • Tuntevski K, Freeman S, Ren X, Hu H, & Yakovenko S. Evaluation of Locomotor Phase Modulation After Cortical Injury in the Rodent Stroke Model. In Neuroscience 2016 (Society for Neuroscience, November 12-16, 2016, San Diego CA); 
  • Tuntevski K, Hu H, Ren X, Simpkins J, Alway S, Mohamed J. Transient Cerebral Ischemic Stroke Activates Multiple Catabolic Signaling Pathways in Skeletal Muscle. In Experimental Biology 2017 (Experimental Biology. EB, April 22-26, 2017, Chicago IL);

Other Activities/Work Experience

West Virginia - IDeA Network of Biomedical Research Excellence (WV-INBRE) Summer Intern (two terms) – West Virginia University School of Medicine Department of Microbiology, Immunology and Cell biology (Morgantown WV)

  • June - August 2010 and June – August 2011

Department of Microbiology, Immunology and Cell Biology (WVU School of Medicine, Morgantown WV) in collaboration with the WVU Nanotechnology Sensing Advances in Field and Environment (NanoSAFE, formerly WVNano – Morgantown, WV) Research Technician

  • Development of PCR-based methods for the detection of invasive Aspergillosis (In-house, qPCR, and applications of fragment size detection for optimization of microfluidic separation methods)
  • March 2013 – August 2013

University of Charleston (Charleston WV), Communications Resource and Academic Success Center (CRC/ASC)

  • Tutor - English, ESL, Biochemistry
  • October 2009 - December 2012

EXP., DEVICES, LABORATORY ROTATIONS & OTHER LABORATORY EXPERIENCE

September 2016 – December 2018

  • Sarcopenia induced by transient brain ischemia and determination of markers of atrophy in the rodent model of transient middle cerebral artery occlusion (MCAO);

September 2013 – September 2017

  • Middle cerebral artery occlusion in rodents – Koizumi and Longa methods (training at the Rodent Surgery Core at West Virginia University School of Medicine performed by Xuefang Ren and Heng Hu); 2015 - 2017
  • Design and optimization of computer-based simulations of neuromechanical models of mammalian locomotion; 2013 - 2016
  • Design and 3D printing of implants for electrophysiological recording; 2013 - 2016 
  • OpenSim Workshop on MATLAB API for real-time closed-loop OpenSim simulations (University of Stanford, Stanford CA); 2015
  • Statistical analyses – Principal Component Analysis (PCA) and Non-negative matrix factorization (NNMF); 2013 - 2016 
  • Optimization of implantation surgeries for chronic electromyographic recording in rodents; 2013 - 2016 
  • Organotypic culturing of rodent superior olivary complex explants; April 2014
  • Analysis of distribution of proinflammatory cytokines in the brain cortex after peripherally induced neuroinflammation by viral mimics; January 2014
  • Reverse transcription PCR (rtPCR) for the detection of RNA subspecies; January 2014 
  • Quantitative PCR (qPCR) for detection of A. fumigatus collagen-like gene fragments; September 2013
  • Exogenous expression of Streptococcus pyogenes collagen-like proteins and protein isolation from the E. coli lacO-ampR transfection plasmid; July 2011
  • Exogenous expression of Aspergillus fumigatus genes in the E. coli lacO-ampR transfection plasmid; 2011 & 2013
  • Polymerase chain-elongation reactions (PCR - end-point and quantitative, qPCR) 2013 - 2018 
  • Western blotting of muscle lysates for the detection of markers of atrophy; 2016 - 2018
  • Co-immunoprecipitation; 2016 - 2018
  • Statistical analysis through data mining (MATLAB - intermediate knowledge); 2013 - 2019

PROGRAMMING, DATA ACQUISITION, and 3D DESIGN

  • MATLAB (MathWorks, Waltham, MA) – intermediate experience; 2013 - 2016
  • Chronic in vivo recording and stimulation of neuromuscular function through the Grapevine system (Ripple, Salt Lake City, UT); 2015 - 2016
  • Xippmex (Ripple, Salt Lake City, UT) 2015 - 2016
  • BLAST (NCBI BLAST and PSI-BLAST, Public Domain) and Pfam Protein Families Database (Sanger Institute) bioinformatics; 2010 - 2013
  • Bio-Rad CFX96 Touch Real-Time PCR Detection System; 2011 - 2013
  • SketchUp Pro (SketchUp, Trimble Inc., formerly Google SketchUp) – advanced experience; 2013 - 2016
  • Confocal microscopy - ZEISS LSM 5 Series Reader and Image Browser for Confocal Microscopy (Carl Zeiss Microscopy GmbH, Jena, Germany); 2013 - 2018
  • Confocal microscopy - ZEISS LSM 510 (Carl Zeiss Microscopy GmbH, Jena, Germany); 2013 - 2018
  • Leica Cryostat CM-3050 (Leica Microsystems, Wetzlar, Germany); 2013 - 2018
  • VF-200 slicer (Precisionary Instruments, Greenville NC, USA); 2013
  • ImageJ (Wayne Rasband, Public Domain); 2017 - 2018
  • Adobe Creative Suite CC2018 (Photoshop и Illustrator, Adobe Systems Inc. San Jose, CA); 2017 - 2018
  • Type A Machines Series 1 3D printer – intermediate to advanced experience; 2013 - 2016
  • Varian CP-3800 Gas Chromatograph/Varian Saturn 2200 Mass Spectrometer (UC - CHEM362 and CHEM412) 2012

NEURAL and MUSCLE PHYSIOLOGY

  • Advanced chronic extracellular and intramuscular recording - Grapevine Trellis System (Ripple, Salt Lake City, UT, USA); 2013 - 2016
  • Dynamometric and force-output analyses of muscle physiology on mouse hindlimb flexors and extensors – 300C dynamometer and DMI Software (Aurora Scientific, Aurora Ontario, Canada); 2016 - 2018
  • Numerical and cross-sectional analyses on muscle fiber type and morphology; 2016 - 2018

AWARDS and HONOURS

  • Dean’s List at the University of Charleston for outstanding grade point average; January 2010 – December 2012 
  • Runner-up (2nd Place) Undergraduate Researcher of the Year awarded by the West Virginia Higher Education Policy Commission at the Science, Technology and Research (STaR) Symposium in Huntington, WV; September 2010
  • Recipient of the Presidential Scholarship at the University of Charleston, WV; August 2009 – December 2012
  • Advanced Placement Program Scholar with Distinction; June 2009
  • Golden Seal, President’s Education Awards Program for Outstanding Academic Excellence, awarded by US President Barack Obama; June 2009
  • Golden Seal, President’s Education Awards Program for Outstanding Academic Excellence, awarded by US President George W. Bush; June 2008
  • “Boris Trajkovski” International Foundation Scholarship for four-year education at NOVA International Schools – Skopje, Macedonia. September 2005 - June 2009

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