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Denis Garoli

Visiting scientist


Via Morego 30
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Born in Padova , get a master Degree in Physics at Padova University. I awarded a PhD in Space Science and Technology at University of Padova with a thesis on the development of innovative technologies for the environmental conservation. In 2011 I get a Degree in Biotechnology at Padova University.
Between 2007 and 2009 I mainly worked on material science R&D for space applications also taking part to a Space Agency’s project (during my collaboration with Media-Lario Technologies – see D. Garoli et al. After this first industrial experience I back to academic research starting my collaboration with Laboratory for Nanofabrication of Nanodevices (LaNN - Venetonanotech) and University of Padova in Padova where I was scientist in charge with Clean Room facilities for nanofabrication. There I extended my view from coatings and material-science to plasmonics and nanotechnologies focusing on nanofabrication and optical applications (see for example D. Garoli et al.;

In the end of 2014 I moved to Istituto Italiano di Tecnologia (IIT), where I’m working as researcher. In 2015 I proposed the idea and write the winning Horizon 2020 FET project ProseqO (co-coordinator and main WP leader within two italian partners – rank #1 among more than 900 proposals): PROtein SEQuencing using Optical single molecule real-time detection. The project regards the development of new technologies for single molecule detection and manipulation with the final goal of DNA/RNA and protein sequencing. Within this project I also started a continuous collaboration with the Biotech company AbAnalitica in Padova (partner in FET project) where I coordinate the ProseqO project and supports the R&D activities. The main results of the research during these years regard the design, fabrication and test of innovative plasmonic devices for several applications, from material science, to biosensing and optical beam manipulation (see list of publication below). During the last two years I mainly focused my activities on nanopore technology for single molecule detection and significant results of the work are now published or under review in high level journals.


Single molecule spectroscopy nanopores 2D materials plasmonics


Horizon 2020 FET Open ProseqO (2016 - 2019) - Coordinator

The project regards the development of new technologies for single molecule detection and manipulationwith the final goal of DNA/RNA and protein sequencing. In particular, the advent of analytical techniques withextremely low limits of detection has led to dramatic progresses in the field of nucleic acids sequencing. Despitethe development of next generation sequencing platforms, the current genome sequencing task remains formidable, and revolutionary advances in DNA sequencing technology are still demanded. Another technological revolution would be a fast and reliable sequencing of proteins, which are the primary actors invirtually all life processes and are coded by DNA sequences known as genes.

Selected Publications

List of IIT publications (2015 - 2019):

  1. Garoli*, T. Ongarello, P. Zilio, M. Carli, and F. Romanato, “Nanofocusing on circular distributed tapered metallic waveguides by means of plasmonic vortex lenses”, Applied Optics, Vol. 54, 1 (2015)
  2. Garoli*, G. Ruffato, P. Zilio, E. Calandrini, F. De Angelis, F. Romanato and S. Cattarin, “Nanoporous Gold Leaves: preparation, optical characterization and plasmonic behavior in the visible and mid-infrared spectral range”, Optical Materials Express Vol. 5, 2246 (2015).
  3. Garoli*, G. Della Giustina, “Directly patternable high refractive index ferroelectric sol-gel resist”, Materials Chemistry and Physics 164, 63-70 (2015).
  4. Garoli*, L. Lovato, G. Della Giustina, M. Oliveiro, M. Francardi, E. Zanchetta, G. Brusatin, and F. De Angelis, “Directly nanopatternable nanoporous Titania – application to cell growth engineering”, Microelectronic Engineering, Volume 155, 2 April 2016, Pages 102-106
  5. Garoli*, P. Zilio, Y. Gorodetski, F. Tantussi, And F. De Angelis, “Optical vortex beam generator at nanoscale level”, Scientific Reports 6:29547 (2016).
  6. Cerea, D. Garoli*, P. Zilio, M. Dipalo, E. Calandrini, A. Jacassi, V. Caprettini, M. G. Pelizzo and F. De Angelis “Modified three-dimensional nanoantennas for infrared hydrogen detection”, Microel. Eng. Volume 162, 16 August 2016, Pages 105-109 (2016).
  7. Garoli, P. Zilio, Y. Gorodetski, F. Tantussi, And F. De Angelis, “Beaming of helical light from plasmonic vortices via adiabatically tapered nanotip”, Nano Lett., 2016, 16 (10), pp 6636–6643.
  8. J. Corso, A. Martucci, M. Bazzan, P. Zuppella, D. Garoli, M. G. Pelizzo, “In-situ real-time investigation of hydrogen-induced structural and optical changes in palladium thin films”, Journal of Alloys and Compounds (2017).
  9. Garoli, E. Calandrini, A. Bozzola, S. Cattarin, S. Barison, M. Ortolani, A. Toma, and F. De Angelis, “Boosting energy transfer in the infrared range in 3D nanoporous antennas”, Nanoscale, 9, 915 (2017).
  10. Garoli, P. Zilio, F. De Angelis, and Y. Gorodetski, “Anomalous light helicity emitted by accelerating singular plasmonic mode”, Nanoscale 2017.
  11. Maccaferri, Y. Gorodetski, P. Zilio, F. De Angelis, and D. Garoli*, “Magnetoplasmonic Vortex Lens for Active Control of Transmission and Helicity of Nano-Structured Optical Beams”, APL (2017).
  12. Mancini, V. Giliberti, A. Alabastri, E. Calandrini, F. De Angelis, D. Garoli*and M. Ortolani, "Thermoplasmonic Effect of Surface Enhanced Infrared Absorption in Vertical Nanoantenna Arrays", Journal of Physical Chem. C. (2018)
  13. Ardini, Jian-An Huang, C. S. Sánchez, V.Caprettini, N. Maccaferri, G. Melle, G. Bruno, D. Garoli*, F. De Angelis, "Live Intracellular Biorthogonal Imaging by Surface Enhanced Raman Spectroscopy using Alkyne-Silver Nanoparticles Clusters", Scientific Reports (2018)
  14. Garoli, E. Calandrini, M. Ortolani, A. Bozzola, A. Toma, S. Cattarin, and F. De Angelis, "Tunable, fractal plasmonic material based on nanoporous gold for infrared applications", ACS Photonics (2018)
  15. Vallani, G. Valenti,M. Maccaccio, L. Mattarozzi, S. Barison, D. Garoli, S. Cattarin, F. Paolucci,  "Coreactanct Electrochemiluminescence at Nanoporous gold electrode", Electroch. ACTA (2018)
  16. Garoli*, D. Mosconi, E. Miele, N. Maccaferri, M. Ardini, G. Giovannini, S. Agnoli and F. De Angelis, "Hybrid Plasmonic Nanopore base on controlled integration of MoS2 flakes on metallic nanoholes", Nanoscale  (2018)
  17. Ponzellini, X. Zambrana-Puyalto, N. Maccaferri, L. Lanzanò, F. De Angelis  and D. Garoli*, "Plasmonic Zero Mode Waveguide for enhanced confined fluorescence emission", Nanoscale  (2018)
  18. Dipalo , G. Melle , L. Lovato , A. Jacassi , F. Santoro , V. Caprettini , A. Schirato , A. Alabastri , G. Bruno , D. Garoli , F. Tantussi and F. De Angelis, "Plasmonic Meta-Electrodes allow intracellular recordings at network level on high density CMOS-multi-electrode arrays", Nature Nanotech (2018)
  19. Mosconi, G. Giovannini, A. Jacassi, P. Ponzellini, N. Maccaferri, P. Vavassori, M. Dipalo, F. De Angelis, S. Agnoli, and D. Garoli* “Controlled integration of MoS2 flakes on metallic nanopores by means of electrophoretic deposition”, ACS Omega  (2019)
  20. M. Ortolani, et al.  “Geometrical Scaling of Ultrafast Temperature Dynamics in Nanoporous Gold”, PRB(2019).
  21. Mancini, V. Giliberti, A. Alabastri, E. Calandrini, F. De Angelis, D. Garoli* and M. Ortolani,"Surface Thermal Gradients Activated by Enhanced Molecular Absorption in Mid-infrared Nanoantenna Arrays", APL  (2019).
  22. A. Petreto, M. Cardoso Dos Santos, O. Lefebvre, G. Ribeiro Dos Santos, P. Ponzellini, D. Garoli, F. De Angelis, M. Ammar, N. Hildebrandt, "Material-independent surface conjugation of FRET-dye pairs on silanized silica layers", ACS Omega  (2018)
  23. D. Mosconi, P. Till, L. Calvillo, T. Kosmala, D. Garoli, A. Martucci, S. Agnoli and G. Granozzi, "Promotion of the hydrogen evolution kinetics by Ni-doping of 3D-structured MoS2 in acid and alkaline media", Nanoscale under review (2019).
  24. X. Zambran-Puyalto, N. Maccaferri, P. Ponzellini, G. Giovannini, F. De Angelis, and D. Garoli* "Site-selective functionalization of plasmonic nanopores for enhanced fluorescence emission and Förster Resonance Energy Transfer", Nanoscale Advances (2019)
  25. Jian-An Huang, M. Z. Mousavi, Y. Zhao, A. Hubarevich, F. Omeis, G. Giovannini, M. Schütte, D. Garoli*, F. De Angelis, "Single-Molecule SERS Detection of DNA Bases by Controllable Trapping of Single Nanoparticles in Plasmonic Nanoholes", Nature Communications under review (2019)
  26. G. C. Messina, X. Zambrana-Puyalto, C. A. Gonano, N. Maccaferri, D. Garoli, F. De Angelis, "

    Controlled two-state switchable thermoplasmonic tweezers for dynamic manipulation of nano-objects based on vertical gold nanochannels.", Submitted to ACS Nano (2019).
  27. D. Garoli, E. Calandrini, G. Giovannini, A. Hubarevich, F. De Angelis, "High sensitivity Nanoporous platform for plasmonic sensing", Nanoscale Horizon  (2019)
  28. P. Ponzellini, G. Giovannini, S. Cattarin, R. Proietti Zaccaria, S. Marras, M. Prato, A. Schirato, F. D’Amico, E. Calandrini, F. De Angelis, W. Yang, J. Haijun, A. Alabastri, and D. Garoli*, "Metallic nanoporous Aluminum/Magnesium Alloy for UV enhanced spectroscopy", JPCC  (2019)
  29. E. Calandrini, G. Giovannini, D. Garoli*, "3D nanoporous antennas as a platform for high sensitivity IR plasmonic sensing", Optics Express (2019)
  30. X. Zambrana-Puyalto, P. Ponzellini, N. Maccaferri, E. Tessarolo, M. G. Pelizzo, W. Zhang, G. Barbillon, G. Lu, and D. Garoli*, "Hybrid metal-dielectric Zero Mode Waveguide for enhanced single molecule detection", Chem Comm. (2019)
  31. D. Garoli*, Y. Yamazaki, N. Maccaferri, M. Wanunu, "Plasmonic nanopores for single molecule detection and manipulation towards sequencing applications", Submitted to Nano Letters (2019)
  32. A. Nana Kaya, J. Cunha, T. Guo, A. Toma, D. Garoli, T. Wang, S. Juodkazis, D. Cojoc, R. Proietti Zaccaria, "Novel plasmonic nanocavities for optical trapping assisted biosensing applications", Submitted to ACS Photonics (2019).


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I numeri di IIT

L’Istituto Italiano di Tecnologia (IIT) è una fondazione di diritto privato - cfr. determinazione Corte dei Conti 23/2015 “IIT è una fondazione da inquadrare fra gli organismi di diritto pubblico con la scelta di un modello di organizzazione di diritto privato per rispondere all’esigenza di assicurare procedure più snelle nella selezione non solo nell’ambito nazionale dei collaboratori, scienziati e ricercatori ”.

IIT è sotto la vigilanza del Ministero dell'Istruzione, dell'Università e della Ricerca e del Ministero dell'Economia e delle Finanze ed è stato istituito con la Legge 326/2003. La Fondazione ha l'obiettivo di promuovere l'eccellenza nella ricerca di base e in quella applicata e di favorire lo sviluppo del sistema economico nazionale. La costruzione dei laboratori iniziata nel 2006 si è conclusa nel 2009.

Lo staff complessivo di IIT conta circa 1440 persone. L’area scientifica è rappresentata da circa l’85% del personale. Il 45% dei ricercatori proviene dall’estero: di questi, il 29% è costituito da stranieri provenienti da oltre 50 Paesi e il 16% da italiani rientrati. Oggi il personale scientifico è composto da circa 60 principal investigators, circa 110 ricercatori e tecnologi di staff, circa 350 post doc, circa 500 studenti di dottorato e borsisti, circa 130 tecnici. Oltre 330 posti su 1400 creati su fondi esterni. Età media 34 anni. 41% donne / 59 % uomini.

Nel 2015 IIT ha ricevuto finanziamenti pubblici per circa 96 milioni di euro (80% del budget), conseguendo fondi esterni per 22 milioni di euro (20% budget) provenienti da 18 progetti europei17 finanziamenti da istituzioni nazionali e internazionali, circa 60 progetti industriali

La produzione di IIT ad oggi vanta circa 6990 pubblicazioni, oltre 130 finanziamenti Europei e 11 ERC, più di 350 domande di brevetto attive, oltre 12 start up costituite e altrettante in fase di lancio. Dal 2009 l’attività scientifica è stata ulteriormente rafforzata con la creazione di dieci centri di ricerca nel territorio nazionale (a Torino, Milano, Trento, Parma, Roma, Pisa, Napoli, Lecce, Ferrara) e internazionale (MIT ed Harvard negli USA) che, unitamente al Laboratorio Centrale di Genova, sviluppano i programmi di ricerca del piano scientifico 2015-2017.

IIT: the numbers

Istituto Italiano di Tecnologia (IIT) is a public research institute that adopts the organizational model of a private law foundation. IIT is overseen by Ministero dell'Istruzione, dell'Università e della Ricerca and Ministero dell'Economia e delle Finanze (the Italian Ministries of Education, Economy and Finance).  The Institute was set up according to Italian law 326/2003 with the objective of promoting excellence in basic and applied research andfostering Italy’s economic development. Construction of the Laboratories started in 2006 and finished in 2009.

IIT has an overall staff of about 1,440 people. The scientific staff covers about 85% of the total. Out of 45% of researchers coming from abroad 29% are foreigners coming from more than 50 countries and 16% are returned Italians. The scientific staff currently consists of approximately 60 Principal Investigators110 researchers and technologists350 post-docs and 500 PhD students and grant holders and 130 technicians. External funding has allowed the creation of more than 330 positions . The average age is 34 and the gender balance proportion  is 41% female against 59% male.

In 2015 IIT received 96 million euros in public funding (accounting for 80% of its budget) and obtained 22 million euros in external funding (accounting for 20% of its budget). External funding comes from 18 European Projects, other 17 national and international competitive projects and approximately 60 industrial projects.

So far IIT accounts for: about 6990 publications, more than 130 European grants and 11 ERC grants, more than 350 patents or patent applications12 up start-ups and as many  which are about to be launched. The Institute’s scientific activity has been further strengthened since 2009 with the establishment of 11 research nodes throughout Italy (Torino, Milano, Trento, Parma, Roma, Pisa, Napoli, Lecce, Ferrara) and abroad (MIT and Harvard University, USA), which, along with the Genoa-based Central Lab, implement the research programs included in the 2015-2017 Strategic Plan.