Because of their non-invasive effect on living systems, light microscopy and spectroscopy have been the workhorses for studies of structures and functions at the cellular, sub-cellular and molecular levels for almost a century.
However, many challenging questions of molecular and cellular biology remain beyond their conventional capabilities. The temporal and spatial resolutions, the labeling and contrast methods of conventional microscopy and spectroscopy pose fundamental limits for investigating the smallest and fastest puzzling mysteries of life.
The core research of our group is the design, development and validation of novel optical and analytical tools that allow the modern biologists to peer inside living cells and organisms with unprecedented temporal-spatial resolutions and minimal invasivity.
This goal can be achieved only working across many disciplines, from physics to engineering, from computer science to biology. Our projects want to synergicaly integrate novel contrast imaging mechanisms, labeling probes, optical architectures and computational approaches that can be readily adopted by IIT's biologists and researchers all over the world.
Molecular Microscopy and Spectroscopy
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Cainero I., Cerutti E., Faretta M., Dellino G.I., Pelicci P.G., Bianchini P., Vicidomini G., Diaspro A., Lanzano L.
Chromatin investigation in the nucleus using a phasor approach to structured illumination microscopy
Biophysical Journal, vol. 120, (no. 12), pp. 2566-2576
Slenders E., Castello M., Buttafava M., Villa F., Tosi A., Lanzano L., Koho S.V., Vicidomini G.
Confocal-based fluorescence fluctuation spectroscopy with a SPAD array detector
Light: Science and Applications, vol. 10, (no. 1)
Slenders E., Perego E., Buttafava M., Tortarolo G., Conca E., Zappone S., Pierzynska Mach A., Villa F., Petrini E. M., Barberis A., Tosi A., Vicidomini G.
Cooled SPAD array detector for low light-dose fluorescence laser scanning microscopy
Biomimetics, vol. 1, (no. 2)