IIT People Search

Paolo Bianchini

Nanoscopy & NIC@IIT
Research center

His main research activity aims at the biophysical study of biological molecules and macromolecules both in situ and in vitro utilizing almost not perturbative instrumentation and nanostructured model systems. So far, his research deals with the design, realization and utilization of biophysical instrumentation as conventional, confocal and multi-photon fluorescence microscopy, SHG imaging microscopy, FLIM, single molecule imaging, STED nanoscopy. In particular, he developed an original setup for SW 2PE-STED (single wavelength two-photon excitation stimulated emission depletion) super-resolution microscopy and another for SHG (second harmonic generation) combined with 2PEF (2-photon excitation fluorescence) microscopy. He recently applied STED nanoscopy technique to the quantitative study of single molecule diffusion in living cells. Moreover, he is in charge of the scientific management of the Nikon Imaging Center and he is responsible for the partnership with the R&D of Nikon Instruments Japan.

Top Publications
Storti B., Quaranta P., Di Primio C., Clementi N., Mancini N., Criscuolo E., Spezia P.G., Carnicelli V., Lottini G., Paolini E., Freer G., Lai M., Costa M., Beltram F., Diaspro A., Pistello M., Zucchi R., Bianchini P., Signore G., Bizzarri R.
A spatial multi-scale fluorescence microscopy toolbox discloses entry checkpoints of SARS-CoV-2 variants in Vero E6 cells
Computational and Structural Biotechnology Journal, vol. 19, pp. 6140-6156
Jadavi S., Bianchini P., Cavalleri O., Dante S., Canale C., Diaspro A.
Correlative nanoscopy: A multimodal approach to molecular resolution
Microscopy Research and Technique, vol. 84, (no. 10), pp. 2472-2482
Bianchini P., Saggau P., Saggau P., Diaspro A., Diaspro A.
An inertia-free beam scanning device for single-wavelength 2PE-STED nanoscopy
Journal of Physics D: Applied Physics, vol. 53, (no. 32)
Deguchi T., Bianchini P., Palazzolo G., Oneto M., Diaspro A., Duocastella M.
Volumetric Lissajous confocal microscopy with tunable spatiotemporal resolution
Biomedical Optics Express, vol. 11, (no. 11), pp. 6293-6310
Cosentino M., Canale C., Bianchini P., Diaspro A.
AFM-STED correlative nanoscopy reveals a dark side in fluorescence microscopy imaging
Science advances, vol. 5, (no. 6)
All Publications
Cuneo L., Castello M., Piazza S., Nepita I., Bianchini P., Vicidomini G., Diaspro A.
A deep learning method to separate fluorophores based on their fluorescence lifetime
Biophysical Journal, vol. 122, (no. 3), pp. 462a-463a
Sheppard C.J.R., Castello M., Tortarolo G., Zunino A., Slenders E., Bianchini P., Vicidomini G., Diaspro A.
Background Rejection in Two-Photon Fluorescence Image Scanning Microscopy
Photonics, vol. 10, (no. 5)
Zeinab Zeaiter L., Baldini F., Cuneo L., Diab F., Bianchini P., Portincasa P., Vergani L., Diaspro A.
Deciphering the chromatin organization and epigenomics involved in adipocyte differentiation and hypertrophy by multimodal nanoscopy
Biophysical Journal, vol. 122, (no. 3S1)
Usai C., Cainero I., Cuneo L., Mariangeli M., Baldini F., Bianchini P., Diaspro A.
Decrypting the spatial relationship between peripheral chromatin and nuclear lamina in Hutchinson-Gilford Progeria Syndrome using super-resolution microscopy techniques.
Biophysical Journal, vol. 122, (no. 3), pp. 491a
Diaspro A., Bianchini P., Callegari F., Cuneo L., Marongiu R., Le Gratiet A., Mohebi A., Scotto M., Sheppard C.J.R.
Emerging Mueller matrix microscopy applications in biophysics and biomedicine
Rivista del Nuovo Cimento della Societa Italiana di Fisica, vol. 46, (no. 8), pp. 473-519
Review Journal
Awards and Achievements
Bianchini P.
TR35-Young Innovators Italia
Bianchini P.
Frontiers in Physics
Bianchini P.
European Biophysics Journal
Bianchini P.
Microscopy Research and Technique
Bianchini P.
Frontiers in Bioengineering and Biotechnology, Nanobiotechnology
Organized Events
Diaspro A., Bianchini P.
8th NIC@IIT Advanced Microscopy practical workshop
Diaspro A., Bianchini P.
7th NIC@IIT Advanced Microscopy practical workshop
Diaspro A., Sheppard C.J.R., Bianchini P.
Diaspro A., Bianchini P.
6th NIC@IIT advanced practical workshop
Diaspro A., Bianchini P., Giacometti G.
IVSLA International School on Nanoscale Optical Microscopy