Agnese Fragassi is a PhD student in the Laboratory of Nanotechnology for Precision Medicine at Istituto Italiano di Tecnologia (IIT), directed by Prof. Paolo Decuzzi. Her research activities focus on the development of polymeric micro- and nano-particles for delivering different drugs (anti-inflammatory drugs and/or growth factors), in order to combine therapeutic activity and regenerative medicine. She joined IIT in March 2019 as Master Student for doing her thesis work and she achieved her MSc degree in November 2019 at the "G. D'Annunzio" of Chieti-Pescara discussing the thesis "Spherical polymeric nanoconstructs loaded with Docetaxel and Curcumin for the treatment of Neuroblastoma". After graduating, she started an internship in IIT during which she continued to work on the same research line. In November 2020, she started her PhD in Sciences and Technologies of Chemistry and Material at the University of Genoa, under the supervision of Professor Decuzzi.
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Nanotechnology for Precision Medicine
Title: Graduation in Pharmacy
Institute: University 'Gabriele D'annunzio'
From: 2014 To: 2019
- -Cell culturing (cancer cell lines: SH-SY5Y, IMR-32, U-87 MG, MDA-MB 231); - Assessing of in vitro toxicity of empty and drug loaded particles (MTT-assay); - Study of cellular uptake of particles using confocal microscopy analysis.
Synthesis of particles
- -Synthesis of polymeric nanoparticles loaded with anti-inflammatory or chemotherapeutic drugs using a sonication-emulsion technique; - Synthesis of microparticles loaded with anti-inflammatory using the hydrogel template method; - Evaluation of particle size and size distribution using dynamic light scattering (DLS) and Coulter Counter ; - Evaluation of particles biopharmaceutical properties: drug loading efficiency, encapsulation efficiency and drug release profile using spectrophotometric or a liquid chromatographic approach; - Use of Scanning electron microscopy