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Development of an In-Vivo Brillouin Microscope (with application to Protein Aggregation-based Pathologies)
Sommario

The role and importance of mechanical properties of cells and tissues in cellular function, development and disease has widely been acknowledged, however standard techniques currently used to assess them exhibit intrinsic limitations (invasive, lack of 3D capability and of sub-cellular resolution). Recently, Brillouin Microscopy (BM), a type of optical elastography, has emerged as a non-destructive, label- and contact-free method that can probe the viscoelastic properties of biological samples with diffraction-limited resolution in 3D. This led to increased attention amongst the biological and medical research communities. However, due to the long acquisition time (hours), this novel technique has been applied only to fixed samples. One important open challenge is to use this approach to follow the mechano-biological processes in living cells and in real time. Aim of our project is overcome the spectral-imaging acquisacknowledged, however standard techniques currently used to assess them exhibit intrinsic limitations (invasive, lack of 3D capability and of sub-cellular resolution). Recently, Brillouin Microscopy (BM), a type of optical elastography, has emerged as a nondestructive, label- and contact-free method that can probe the viscoelastic properties of biological samples with diffraction-limited resolution in 3D. This led to increased attention amongst the biological and medical research communities. However, due to the long acquisition time (hours), this novel technique has been applied only to fixed samples. One important open challenge is to use this approach to follow the mechano-biological processes in living cells and in real time. Aim of our project is overcome the spectral imaging acquisition time limitation by applying the heterodyne detection scheme, allowing the collection of viscoelastic properties in living sample at a sub-second timescale. We will attempt solving specific physio-pathological open problems in biomedicine for the first time.

LogoEnteFinanziatore HE
Persone coinvolte
Giancarlo Ruocco
Giancarlo Ruocco
Nanotechnologies for Neurosciences
Gian Gaetano Tartaglia
Gian Gaetano Tartaglia
RNA Systems Biology
Informazioni
IVBM-4PAP
Acronimo
IVBM-4PAP
Data inizio
01/03/2023
Data fine
31/08/2026
Ruolo
Coordinator
Funds
European
Budget
Total budget: 1.629.372,71€
Total contribution: 1.629.372,71€
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