Thermostabilized Amadoriase I from aspergillus fumigatus

The invention relates to a new-engineered Amadoriase enzyme with increased thermostability with respect to the wild-type enzyme. The enzyme here obtained can be useful as deglycating agent in the food industry processes, where high temperature is necessary (e.g. milk pasteurization at 75-85°C for 10-15 seconds). The mutant is active up to 80-85°C differently from the wild type enzyme, which is active only at T < 50°C. The new enzyme is obtained by using a combined computational and experimental approach. A computational method is used to identify amino acids that can be mutated to cysteines in order to introduce one or two disulphide bonds (i.e. cysteine-cysteine covalent bond) in the structure of Amadoriase I. Then experiments proving the thermostability of the enzymes are provided to select the more efficient mutants.

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2,6-disubstituted 4-amino-pyrimidines and 4-amino-triazines as treatments for processes driven by RhoJ/Cdc42 signaling, including melanoma, other cancers, retinal disorders, and cardiomyopathies.

The invention relates to 2-heterocycloalkyl-6-aryl-4-aminopyrimidine and 2-heterocycloalkyl-6-aryl-4-aminotriazine derivatives. The invention also relates to the use of said compounds, alone or in combination with other current anticancer drugs, in the treatment melanoma and/or growth of tumors in mammals. In particular, the here disclosed 2,6-disubstituted-4-amino-pyrimidines derivatives are potent inhibitors of RhoJ, and other members of the Cdc42 family, interaction with Pak1

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5-carboxamide derivatives of 2-thiobarbituric acid as type II topoisomerase inhibitors and their use as medicaments

The invention relates to a class of 5-carboxamide-2-thiobarbituric acid derivatives able to inhibit human type II topoisomerase (topo II) enzyme. The invention also relates to the use of said 5-carboxamide-2-thiobarbituric acid derivatives for the pharmacological inhibition of topo II in the treatment of a variety of pathophysiological conditions, especially in the treatment of cancer. The compounds subject-matter of the present invention proved to be potent inhibitors towards topo II and, at the same time, showed promising results in blocking the proliferation of cancer cells.

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Compounds and compositions for the treatment of cystic fibrosis

The invention relates to a first class of compounds, pharmaceutically acceptable salts or solvates thereof. It further discloses a pharmaceutical composition comprising the compounds and their uses. In particular, the compounds of the invention act as correctors of the mutated protein that is responsible for cystic fibrosis, i.e. Cystic Fibrosis Transmembrane conductance Regulator (CFTR)

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Compounds and compositions for the treatment of cystic fibrosis

The invention relates to a second class of compounds, pharmaceutically acceptable salts or solvates thereof. It further discloses a pharmaceutical composition comprising the compounds and their uses. In particular, the compounds of the invention act as correctors of the mutated protein that is responsible for cystic fibrosis, i.e. Cystic Fibrosis Transmembrane conductance Regulator (CFTR)

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Functionalized benzimidazole and close derivatives as acide ceramidase inhibitors and their use as medicaments

The present invention relates to functionalized benzimidazole derivatives as acid ceramidase (AC) inhibitors. In particular, the present invention concerns acid ceramidase inhibitors compounds, pharmaceutical compositions containing them and methods for preparing them.

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Synthesis of thermo-responsive iron oxide nanoparticles with high specific absorption rates as platform for cancer treatment

The invention deals with a process for the synthesis of magnetic nanoparticles coated with a thermo or pH responsive polymer using the so-called surface-initiated polymerization. The process comprises a radical polymerization step of a monomer or co-monomers susceptible of forming a thermo-polymer or pH responsive copolymer in a solution including magnetic nanoparticles functionalized at their surface with a polymerization initiator. The process of the invention allows functionalizing strongly interactive cubic iron oxide nanoparticles in an individual state, thus retaining the outstanding heating performances.

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Water-based process for converting vegetables pomace in bioplastic films

The process of the invention converts cellulose-rich vegetables, in particular originating from pomace, into bioplastic films. The resulting material is a composite film wherein cellulose with different degrees of hydrolization, pectins and other soluble matters like sugars are the matrix and water-insoluble materials (e.g. lignin) are homogeneously dispersed into the film. The conversion of dried waste into a plastic film is carried out dispersing the dried waste in an acidic water-based solution, having HCl in a concentration between 0,1 and 5% by weight, at room temperature and stirring for few hours. Upon casting the whole solution and evaporating the solvent, a bioplastic film like those shown in pictures A-D is obtained.

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Neuro-specific viral vector for gene therapy to treat cognitive impairment in Down syndrome by RNA interference

The invention deals with the use of artificial micro RNA (amiR) to reduce the expression of NKCC1 protein (encoded by SLC12A2 gene), which is involved in the pathogenesis of several diseases via regulation of intracellular chloride concentration. The invention is based on recent studies showing that NKCC1 is overexpressed in the brains of patients with Down Syndrome and pharmacological treatment with the inhibitor Bumetanide restores synaptic plasticity and cognitive impairment in mouse models. However, several data indicated that a life-long treatment with Bumetanide would be required, thus potentially inducing indesirable excessive diuresis and related side effects of chronic imbalance in a number of electrolytes. The invention therefore provides an engineered system to achieve neuron-specific expression of specific amiR against NKCC1 by using a human Synapsin promoter to drive transgene expression.

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Nano-carrier loaded with an exocytosis inhibitor and an active ingredient

The invention relates to a new drugs carrier system of based on a nano-capsule containing a lysosomal exocytosis inhibitor and a drug. The inhibitor is used to stop the cellular lysosomal exocytosis process resulting in an intracellular entertainment of nanoparticles and in an extended release of the carried drug.

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Cooperative primers PCR (coop. PCR)

The invention relates to a method for the simultaneous identification and amplification of single nucleotide polymorphisms (SNPs). This method, called COOperative Primers PCR (CooP PCR), is based on the use of short PCR primers that cooperatively hybridize on the SNP site on the template DNA, highly enhancing the SNP discrimination efficiency. This allows highly specific PCR amplification only of the SNP variant searched for. The assay is low-cost, as it is based on standard PCR primers, and universal, as it can be applied to any SNPs, irrespective of its genomic context.

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Gold core hollow nanostructures

Metal nanoparticles for health applications have not translated into market because they accumulate in organism after the designed action. In order to overcome this issue the invention discloses an innovative kind of multifunctional nanoparticles that maintain the features of metal nanoparticles, and biodegrade to renal clearable building blocks in few days.

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Tumor treatment

The present invention shows that the pyrimidine analog, Azaribine, can be used as a single agent to induce p53-dependent death in tumor cells. Furthermore, in the invention we propose that combined treatment with Azaribine and the MDM2 antagonist, Nutlin-3A (the chemical precursor of RG7112) induces synergistic death in cancer cells expressing wild type p53. This is supported by experimental data.

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Process For The Preparation Of Graphene Dispersions

The invention relates to the field of foldable conductive biomaterials suitable to be employed in several flexible electronic devices. In particular, the aim of the invention is to provide a fast, green and easy scalable method to produce a versatile, flexible, robust, low cost and easily disposable fibrous bio-composite which can be employed for different applications, from electromagnetic interference (EMI) shielding to electrodes for electromyography (EMG) and finally to foldable and wearable electronics.

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Bi-stable scalable actuation mechanism based on electromagnetic adhesion

The invention describe a method based on a “miRNA Pool” to generate neurons from adult neural stem cells, while preventing generation of glia. The present invention could be applied to prevent age-dependent loss of neurogenesis, or to avoid the pathological generation of undesirable cells such as activated glia upon trauma, epilepsy or cell transplantation. Finally, another application of the present invention is related to the treatment of glioblastoma.

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Method for the synthesis of carbon nano-onions

The invention relates to a method to obtain carbon nanostructures which a structure of onion. The carbon nano-onions here presented show graphitic multilayer morphology which allows to be used to different bio application as in vivo imaging, catalysis, gas storage electromagnetic shielding. The method object of the invention is inexpensive, easy to control and adeguate to be scalable

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4-amino-2-pyrido bicyclic pyrimidines as type ii topoisomerase inhibitors and their use as medicaments

The invention relates to compounds (i.e. 4-amino-pyrido[4,3-d]pyrimidine and 4-amino-quinazoline derivatives, collectively termed 4-amino-2-Pyrido bicyclic pyrimidines)that inhibit human type II topoisomerase (topoII) enzyme. Pharmacological inhibition of topoII can be used to treat a variety of pathophysiological conditions, especially cancer. The here proposed 4-amino-2-Pyrido bicyclic pyrimidines are potent inhibitors towards topoII and, at the same time, these compounds show promising results in blocking the proliferation of cancer cells. Moreover, the 4-amino-2-Pyrido bicyclic pyrimidine scaffold is very drug-like and allows great chemical diversity, for this reason theseseries of compounds could constitute a highly promising foundation for further drug development.

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Notch inhibitors

The invention relates to the identification of the natural molecule 3,4,2′,4′-Tetrahydroxychalcone and to the generation of one related sintetic derivative. Both molecules present an inhibitory activity of the Notch signaling

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Piperazinyl Methanone NAAA Inhibitors

Piperazinyl Methanone NAAA Inhibitors

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Graphene biosensor for the recognition of exosomes from human fluids

The invention relates to a device able to recognize, purify, and analyze exosomes from biological fluids. It is composed by a surface-modified graphene that can be supported on different substrates, including SiC or glass. The surface is modified with antibodies or aptamers which allow to bind the target. Thanks to this modification, exosomes are recognized with a high degree of specificity. After/during the recognizing step, the device can be analyzed by optical or electrical methods such as microscopy or electrical conductivity measurements, respectively.

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Method for dynamical configurational sampling of molecular interactions

The invention relates to a method to facilitate the acceleration of the configurationally sampling of molecular interaction in molecular dynamics (MD) simulation. This method makes use of an auxiliary (Yukawa-like) screened electrostatic interaction potential acting between the subsets of a molecular system the interaction of which one wants to sample. This potential can be easily implemented in MD plugins and smears the interaction over all the atoms of the two subsets, leading to a concerted global movement.

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Modulation of cognitive disfunctions with dopaminergic drugs

The invention relates to a substance selected from the group consisting of agonists and antagonists of the dopamine D2 receptors, for use in modulating cognitive dysfunctions in a subject who bears a functional genetic variation in the DTNBP1 gene capable of lowering the levels of the dysbindin-1 protein.

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Multi-channel modular neural stimulator

The invention relates to a stimulation unit for a neuromuscular system comprising a plurality of terminals configured to be electrically coupled to a corresponding plurality of electrodes of a stimulation matrix configured to stimulate a portion of neuromuscular system, a signal generator programmable in order to generate a stimulation currents on each terminal and to detect signals. The device could control whatever number of the said electrodes which could be of any shape. The device could also control the stimulation time of each electrodes forming complex stimulation patterns.

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Compounds for treatment of neurodegenerative disorders

The invention relates to Compounds and pharmaceutical compositions containing the same. It further relates to their use in the prevention or treatment of central nervous system diseases or disorders, in particular, cognitive, neurodegenerative or neuronal diseases or disorders.

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Kinetics of protein-ligand unbinding

A computational methodology to estimate the unbinding kinetics inherent in biomolecular interaction, also tested in the case of protein-ligand binding, is provided. This method couples smoothed potential molecular dynamics simulations with a statistical treatment. A method is further provided for use on systems of pharmacological interest, including those for which kinetic experimental data are available, including heat shock proteins (HSP70 and HSP90) that bind two distinct classes of ligands and the Adenosine A2A G-Protein Coupled Receptor in complex with congeneric inhibitors. The method provides the ability to rank ligands in consistent agreement with experimental kinetic data and to provide residence time estimates that correlate well with corresponding experimental measurements obtained from surface plasmon resonance experiments.

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Linearly actuated two degree-of-freedom spherical orienting device for optical elements

The invention relates to a new device for the motorized control of the aiming and scanning motions of a laser beam. The particular conformation of the presented device permits to increase the overall performances in terms of speed and precision of the laser orientation in comparison to the existing devices. Its first potential application is laser microsurgery, similar devices present in prior art are called laser micromanipulator. Therefore, the system could be termed as “Linear actuated Laser Micromanipulator.

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Acid Ceramidase inhibitors, and their use as anti-tumor and anti-inflammatory agents

Anti-tumor and anti-inflammatory agents

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Novel Acid Ceramidase inhibitors, and their use as anti-tumor and anti-inflammatory agents

Novel anti-tumor and anti-inflammatory agents

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Method for producing a totally endogenous skin

The present invention relates to a method for producing a totally endogenous bioengineered tissue including a first layer of connective tissue and a second layer of epithelial tissue, to a tissue equivalent obtained thereby and to a method for determining the effect of a chemical substance or an agent on skin employing the tissue equivalent.

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Fatty Acid Amide Hydrolase Inhibitors based on bicyclic systems

Medicaments

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Modulators for treating an intellectual disability

The present invention relates to a composition for treating an intellectual disability in a subject in need thereof, wherein said composition comprises an effective amount of a modulator of a chloride transporter.

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Liquid encapsulation process

Globular microstructures comprising a liquid core and a solid shell that envelops the core comprising the micro-or nanofiber polymer, preferably obtained by electro-spinning, comprising a hydrophobic polymer or a mixture of polymer with hydrophobic polymers derived from cellulose or polyacrylates; microstructures may have an additional coating of nanoparticles or polymer. The microstructures have applications similar to those of the "liquid marbles" with improved mechanical properties.

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Distal scanning module for the pointing and the displacement of an optical or a medical device

The invention relates to a device that includes: · an elongated structure; an optical apparatus associated with the elongated structure and adapted to be exposed to a portion located within a body cavity of a patient; · an actuating apparatus arranged to control the position of the elongated structure in such a way as to orient the optical device into the body cavity. The elongated structure has a proximal portion and a portion with a deformable extremity that has the tendency to remain and elastically return in default state in which it is normally flexed. The drive apparatus comprises a guided mobile pusher arranged to insist against the said extremity in order to angularly move it from the folded state to a substantially straightened state.

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System for manufacturing a scaffold for a cell culture including a pneumatic device for transferring microparticles, and related scaffold

The invention relates to a system for manufacturing a scaffold for a cell culture including a transferring device that picks a particle from a plurality of particles arranged in a container and places singularly the picked particle in contact with a scaffold support matrix.

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Diarylalkylamine rev-erb antagonists and their use as medicaments

Medicaments

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Dual Inhibitors Of The Fatty Acid Amide Hydrolase Enzyme And Modulators Of The D3 Dopamine Receptor

Medicaments

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Optogenetic tool for multiple and independently addressing of patterned optical windows

The invention relates to a multi‐point light‐delivering device, comprising a waveguide carrying light along a longitudinal axis and including multiple optical windows, through which the carried light is out‐coupled from the waveguide. The waveguide comprises a tapered region along which the optical windows are distributed, wherein each optical window out‐couples a specific subset of propagating modes of the carried light, to which the optical window is matched

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O-alkyl triazolyl carbamates as inhibitors of fatty acid amide hydrolase (FAAH)

FAAH inhibitors

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Biodegradable nanocarrier for drug delivery

The invention relates to a block-made copolymer consisting of two blocks S1 and S2, where S1 is constituted by m(PEG)n where n is an integer between 4 and 50, and S2 is a random copolymer consisting of the monomeric units R1 and R2 where R1 is the monomer unit corresponding to the monomer gvalerolattone and R2 the monomer unit corresponding to the monomer selected from the group consisting e-caprolattone, δ-valerolattone, β-butirrolattone, ε-caprolattame, δ-valerolattame. Is also provided a method for its preparation, its use and a pharmaceutical composition comprising the same.

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Adhesive composition comprising a cyanoacrylate compound with a silyl isocyanate treated silica gel

The present invention relates to an instantaneous adhesive composition which is satisfactorily made thixotropic or gelled and has a rapid setting time and an excellent adhesive strength, said composition being obtained by adding to an α-cyanoacrilate a silica gel treated with a silyl isocyanate.

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Carbamate Derivatives Of Lactam Based N-Acylethanolamine Acid Amidase (NAAA) Inhibitors

NAAA inhibitors

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Amide Derivatives Of Lactam Based N-Acylethanolamine Acid Amidase (NAAA) Inhibitors

NAAA inhibitors

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Heat-Sensitive Nanoparticle System

Heat-sensitive system comprising at least one nanoparticle ableto convert an electromagnetic radiation into thermal energy when said nanoparticle is exposed to an alternating magnetic field, said nanoparticle being bound covalently with at least one thermolabile molecule, said thermolabile molecule being covalently bound with at least one active molecule selected from a fluorophore molecule and a drug, characterised in that said thermolabile molecule comprises an azo -N=N- functional group.

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Probe Kit For Detecting A Single Strand Target Nucleotide Sequence

A system for detecting a single strand target nucleotide sequence comprising: •at least one first nucleic acid probe from 10 to 14 bases, to the 5’ end of which at least one fluorophore is bound; •at least one second nucleic acid probe from 35 to 50 bases, comprising, from the 5’ to the 3’ end •a first segment having a nucleotide sequence complementary to the first nucleic acid probe, •at least one quencher, •a second segment having a nucleotide sequence complementary to at least part of the target nucleotide sequence.

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Combined material including anodic porous alumina and a polymer matrix, and its use for the dental recondition

The present invention relates to anodic porous alumina (APA) in the form of microparticles, characterized in that it contains interconnected through nanopores, and to its use in the preparation of a new composite material, which is useful for example in the field of conservative dentistry. The invention further relates to a process for preparing the nanoporous alumina of the invention in microparticles.Thanks to the mechanical interlace that is established between the microparticles of nanoporous alumina and the polymer matrix, the composite material of the invention does not require the use of any coupling agent, further ensuring excellent properties in terms of resistance, elasticity, biocompatibility and stability over time. In fact, the particular microparticulate form of the nanoporous alumina and the presence of interconnected through holes in each microparticle makes it possible to achieve an almost complete penetration of the polymer matrix into the alumina nanopores. In this manner the two components of the composite material are physically interconnected without there being a need to use any type of chemical coupling agent.

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Method for obtaining ultrastable nano‐emulsions

A new method to obtain ultra-stable emulsions is described. The stability of an emulsion can be strongly improved by associating to a right formulation the right process or better sequences of processes. After coating the emulsion with a thin polymer shell it has to be re-dispersed one or more times to homogenize the formulation which allows stabilities at least higher than 9 months. The developed product is also perfectly biodegradable that is a fundamental requisite for applications in food, cosmetic and pharmaceutical fields with sizes ranging between 50 and 200nm and with good Poly-Dispersion Indexes (PDI below 0.1) as required for these applications.

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Acid ceramidase inhibitors and their use as medicaments

The present invention concerns, in a first aspect, compounds of Formula I, pharmaceutically acceptable salts thereof and pharmaceutical compositions containing such compounds. The present invention also relates to compounds of Formula I for use as acid ceramidase inhibitors, and in the treatment of cancer and other disorders in which modulation of the levels of ceramide is clinically relevant.

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Acid ceramidase inhibitors and their use as medicaments

The present invention relates to compounds of Formula I, its pharmaceutically acceptable salts and it's pharmaceutical compositions containing such compounds. The present invention also relates to compounds of Formula I for use in the treatment of cancer and other disorders in which modulation of the levels of ceramide is clinically relevant.

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Multitarget FAAH And COX Inhibitors And Therapeutical Uses Thereof

The invention features compounds that inhibit the fatty acide amide hydrolase (FAAH), and/or cyclooxygenase 1 (COX1) and/or cyclooxygenase 2(COX2). Certain of the inhibitors are selective for FAAH relative to COXs. Others are selective for COXs relative to FAAH. Some are inhibitors of both FAAH and COXs. Pharmacological inhibition of FAAH and/or COXs can be used to treat a variety of pathophysiological conditions such as inflammation and pain treatment, as well as anxiety, eating disorders, and cardiovascular disorders.

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Novel Lantipeptide

The present invention concerns novel lantipeptide (lanthionine-containing peptide) compounds having general formula (I), a process for their preparation, the key intermediates in said processes, their pharmaceutical acceptable salts and pharmaceutical compositions containing them, as well as their use in the treatment of pain.

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Dual Target Compounds For The Treatment Of Alzheimer’s Disease

Compounds of formula (I) wherein the groups are as defined in the description, are used as medicaments, in particular for the treatment of a disease selected from the group consisting of: cognitive impairment, memory dysfunction, neurodegenerative disorders and related dementia, Alzheimer’s disease, Parkinson’s disease, neuropsychiatric behavior associated with Alzheimer’s disease, pain, depression, attention deficit hyperactivity disorder and for pharmacological addictive substance or intoxicant therapy; and for the neuroprotection from NMDA toxicity.

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Polymeric composite materials with antimicrobial and biodegradable properties

Povidone Iodine (PVPI) is a well known broad spectrum anticeptic for wound treatment and irrigation. PVPI, however, is a very hydrophilic substrance having poor resistance against water. Wound treatments with PVPI are, therefore, short lived. We have developed a simple and inexpensive method to directly incorporate PVPI in alginic (sodium & calcium) polymer matrices to enable its slow and controlled release into infected areas. The process also prolongs antiseptic effects of PVPI considerably. Aqueous PVPI solutions are blended with sodium alginate solutions at any proportion from which films can be cast. Droplets or continuous liquid streams of the blend solutions can be cross-linked in calcium salt solutions to form PVPI encapsulated beads and fibers.

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Automatic passive liquid positioning in microfluidic chips

A complete Lab-On-Chip (LOC), based on a microfluidic piezoelectric chip able to steer surface acoustic waves (SAWs) passively and with minimal losses depending on the position of the fluid microdroplets on the chip. LOCs are one of the most promising technologies in the biomedical field. Miniaturized, portable diagnostic and analysis system are expected to constitute the next-generation tool for medicine and other fields. The liquid handling system is at the base of every LOC. It must enable loading, actuation and precise positioning of very small liquid volumes. LOC technologies are attracting the biomedical market thanks to the new capabilities introduced by the microfluidic chips and by optimized performances in terms of liquid consumption, waste production, scalability and high-throughput analysis.

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Drug release vector

A structure based on 30 nm gold nanospheres conjugated to (bio)molecules by a triazole ring formed through click-chemistry is developed. 30 nm gold nanospheres (AuNs) offer a number of attractive properties such as maximum efficiency in terms of cellular uptake, and an extinction band peaked at 530 nm. The AuNs are coated with an alkyne-modified peptide (CLPFF-Propargylglycine; G), in order to obtain stable bifunctionalized gold colloids with both carboxylic acid and alkyne groups on the metallic surface (AuNsG), so that the system can be easily conjugated with multiple molecular species. The alkyne groups can be readily coupled to azide-probes, such as azide-fluorescein (Fn), by click-chemistry reactions, obtaining the 1,2,3-triazole ring linkage between the metallic nanostructures and the probe. Owing to the field enhancement effect occurring in proximity of the metallic nanostructure for the localized surface plasmon resonance (LSPR) stimulation, this system can effectively release the probe through a 3-photons effect by irradiation with 561-nm laser light. In this way it is possible to control both spatially and temporally the release of a probe internalized in living cells by the use of visible light close to the red part of the spectrum.

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A dynamic brain pressure control for single unit in vivo neural recordings

In the research field of the Cognitive Sciences, the RBCS department has developed compact multi-channel single and multi-unit in vivo neural recording system. This device permits both a very precise electrode positioning with respect of the brain tissue and the application of a finely, dinamically controlled pressure in the recording site. This device allows the surgeon to monitor the pressure exerted by the recording device on the brain tissue while a separate control adjust gradually this pressure, dynamically following and limiting the brain tissue bulging and pulsation. In this way dangerous interruptions of brain blood supply in the recording site due to excessive pressure produced by the recording device are avoided while brain pulsations are reduced.

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A dynamic brain pressure control for single unit in vivo neural recordings

In the research field of the Cognitive Sciences, the RBCS department has developed compact multi-channel single and multi-unit in vivo neural recording system. This device permits both a very precise electrode positioning with respect of the brain tissue and the application of a finely, dinamically controlled pressure in the recording site. This device allows the surgeon to monitor the pressure exerted by the recording device on the brain tissue while a separate control adjust gradually this pressure, dynamically following and limiting the brain tissue bulging and pulsation. In this way dangerous interruptions of brain blood supply in the recording site due to excessive pressure produced by the recording device are avoided while brain pulsations are reduced.

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Three‐Electrode electroporation device

In vivo electroporation makes it possible to render cell membranes temporarily permeable to substances that otherwise would not be able to effectively enter the cell interior. This invention describes a simple and yet very effective redesign of the existing electroporation devices, using three electrodes instead of two. This device presents a new electrode configuration for in vivo electroporation in the nervous system of embryonic mice. The new configuration entails a common bipolar electrode connected to only one polarity and a third new electrode connected to the other polarity.

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Open three‐sided magnet for MRI

In the field of the study of the human being, in particular of the brain activities, MRI provides excellent structural and functional images of the soft tissues, organs and vasculature in any desired spatial plane. This is achieved when the patient is placed inside a powerful magnetic field. The open three-sided magnet, described in this invention, overcomes most of the limitations due to the traditional solenoid design of MRI machines.

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Star‐shaped gold nanoparticles

Method to synthesize in aqueous solution branched gold nanoparticles by which it is possible to control the size and the degree of branching at the same time. The method does not use cytotoxic capping agents, such as CTAB, organic thiol molecules or others. A further coating can also be realized on the surface of branched nanoparticles. It also allows to control with extreme precision the optical properties of the nanoparticles in a broad region of UV-visible and near-ir spectrum. The nanoparticles can be used for application based on the Surface Enhanced Raman Scattering phenomenon, or as Metal Enhanced Fluorescence or Metal Enhanced Chemiluminescence materials. Due to the finely optical absorption in certain region of spectrum, these nanoparticles can be applied also for the therapeutic treatment of neoplastic diseases through a photo-thermal effect.

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Conductive polymer free‐standing nanosheets

This invention realizes free-standing conductive ultra-thin films based on poly(3,4-ethylenedioxythiophene)/poly (styrenesulfonate) (PEDOT/PSS), proposing a fabrication process based on a modified Supporting Layer technique, that provides for the easy production of conductive nanofilms having a very large surface area with typical thickness of tens of nanometres. The free-standing nanofilms can be manipulated, folded and unfolded in water many times without suffering from cracks, disaggregation or from loss of conductive properties. After collecting them onto rigid or soft substrates, they retain their functionality.Possible applications are foreseen in the field of sensing and actuation, as well as in the biomedical field, e.g. as smart substrates for cell culturing and stimulation.

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Biocompatible, Free‐Standing Nanofilms Of Conductive Polymers

The invention is related to the obtainment of free-standing nanofilms of conductive polymers. The method to obtain these films comprises four steps. In the first one there is the sequential deposition of, respectively, a sacrificial material, a layer of an electrolyte, a layer of the conductive polymer and, eventually, a further layer of polyelectrolyte. The second step is a thermal treatment of the multilayer structure; in the third step the sacrificial support is removed and in the last step the free-standing multilayer structure is transferred in solution. Due to their characteristics of flexibility, robustness, adhesion to different substrates and biocompatibility, are particularly useful in biomedical applications, e.g. as support for cell grow.

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Sensing moisture measuring device

A human skin moisture measuring device comprising an interdigitated resistive sensor on a transparent support, an image sensing device, such as a video camera, a CCD device or a C-MOS device, and a lighting device. The device allows evaluating at the same time the moisture of skin and the real surface of skin in contact with the sensor; this last feature can be also used for studies on tactile perception.

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Open‐Structure Magnetic Assembly For Magnetic Resonance Imaging

The invention is related to the generation of magnetic fields, particularly for magnetic resonance imaging (MRI). MRI requires a high strength and uniformity magnetic field over a range of interest and in the case of human subjects it is also necessary that the shape of the magnet be suitable for easy positioning of the subject under examination; the volume occupied by the subject has to be located in a region of homogeneous field, i.e. in a region where the magnetic field exhibits equal intensity and is unidirectional. The invention is able to produce an open structure magnetic assembly characterized by producing a homogeneous magnetic field in an easily accessible region. The principle is to obtain homogeneous magnetic fields by adding magnetic fields originating by different structures.

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Nano‐probes for the electric brain stimulation

From this research, a new family of nano probes have been developed. These probes can be put in contact directly with brain cells and, thanks to their piezoelectric effect, they can perform an electrical stimulation at a single-cell scale.This invention has the outstanding advantage of non-invasiveness, by which all the risks of infection, contamination, hemorrhage, tissue and organ damage, as well as the stress of surgery are avoided for the patient subjected to the electric stimulation treatment.

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Magnetic-Fluorescent Nanobeads

This invention implements a new method of simultaneous detection and separation of biological entities like specific cells, based on magnetic nanobeads of strictly controlled size. The IIT Nano Chemistry research group, has developed nanobeads made of aggregates of iron oxide nanoparticles enwrapped within an amphiphillic polymer to which oligothiophene fluorescents are grafted; their size is selectable in the range 30 to 400 nm. The nanobeds can be designed to exploit both a fluorescent and a magnetic effect, and can be used to target cancer cells. Thanks to the magnetic effect, the can be used to interact with the target and, because of the fluorescent properties, they can be used as reactor in the investigation process.

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Ankle Rehabilitation Platform

The Advanced Robotics department is involved in an interdisciplinary and interdepartmental activity, oriented to human rehabilitation. With this perspective, researchers of the Italian Institute of Technology have developed this Ankle Rehabilitation Platform, a medical device able to accelerate and significantly improve the functional recovery of patients with ankle impairments. The simplicity and compactness of the device together with its redundant characteristic, which allows simultaneous control of position and stiffness, improves the quality of and increase the productivity of ankle physiotherapy delivered by the clinicians. The actuator, core of this rehabilitation device, presents unmatched technical features: • High speed and force output •Backdrivability •Very high power to size ratio •Good power to weight ratio •Long stroke and high position resolution •The actuator is equipped with full state sensing, namely an encoder for position and velocity measurements and an axial load cell mounted on the tip of the moving piston. This allows to control the actuator in either position/velocity or force

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Electric Neurostimulator

The invention relates to an electric neurostimulator, whose electric circuit is located on a single integrated circuit, comprising: an electric stimuli generator; a reader of the signals received from said patient; at least one electrode applied to said patient; said at least one electrode receives said electric stimuli and sends them to said patient; said at least one electrode receives at least a signal from said patient and sends it to said signal reader; switching means for selectively connect said at least one electrode to said electric stimuli generator or said signal reader

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Polymer Microelectrode Array

TMicroelectrode electrophysiology has become a widespread technique for the extracellular recording of bioelectrical signals. To date, electrodes are made of metals or inorganic semiconductors, or hybrids thereof. We demonstrate that these traditional conductors can be completely substituted by highly flexible electroconductive polymers. The bendable, somewhat stretchable, non-cytotoxic and biostable all-polymer microelectrode arrays (polyMEAs) with a thickness below 500 mm and up to 60 electrodes reliably capture action potentials (APs) and local field potentials (LFPs) from acute preparations of heart muscle cells and retinal whole mounts, in vivo epicortical and epidural recordings as well as during long-term in vitro recordings from cortico-hippocampal co- cultures.

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Actuator device for a robotic lower limb and trunk/core rehabilitation machine

A robust and compact actuator device applied in a preferred embodiment to a robotic machine for ankle physiotherapy exercises, rehabilitation in the upright position in monopodalic or bipodalic conditions, spine rehabilitation and core-stability exercises in a sitting position.

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Fiducial marker for stereotassic radiosurgery and production process

Composition in the form of shaped device, for use as a fiducial marker in tissues in the animal body, in radiotherapy and/or radiosurgery comprising a core consisting of a colloidal dispersion of metal nanoparticles and/or oxides or metal salts having X-ray-contrast properties, where said nanoparticles are stabilized with surfactants, polymers or capping agents in a liquid vehicle, and a casing that encapsulates the core polymer, said device having a minimum size of not less than 500 microns and a maximum size not greater than 3000 microns.

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Antimicrobial cellulose acetate – Essential oil natural nanocapsules and uses thereof

The present invention relates to antimicrobial biocompatible nanocapsules constituted of cellulose acetate and lemongrass oil, which maintain its antimicrobial action, for biomedical applications. Said nanocapsules are suitable for safer drug delivering because of their high stability in water due to the lemongrass encapsulation in cellulose acetate nanoparticles by a hemiacetal bond between the aldehydes contained in the essential oil and the OH groups of the polymer. The method for obtaining the antimicrobial nanocapsules comprises a first step where cellulose acetate and lemongrass are dissolved in appropriate amounts of acetone and a second step where they are subsequently mixed with an appropriate amount of water; during this last step the in-situ nanoprecipitation of the product occurs and the size of the nanocapsules can be optimized tuning the acetone/water ratio. Finally the acetone is removed by evaporation in air under stirring and the obtained aqueous nanocapsules can be employed as prepared or as powder which is provided using spray dry technique.

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Method for plasmonic cell poration

The scope of the invention is to allow the access to the inside of living cells without any kind of perturbation to its state. This invention could be used for delivering objects inside the cell after a selectively plasmonic poration, for measuring quantities in the intracellular environment and for obtaining discrimination of intra and extra cellular information.

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Method for producing or treating alginate-based structures by trifluoroacetic acid and its derivatives

This invention describes a method to produce alginate-based micro- and nanostructures, conferring them controlled stability over time in aqueous media and adjustable biodegradability. To this end, trifluoroacetic acid (TFA) and its derivatives are used.

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Gold Seeds Embedded in Hollow Silica Nanoparticles

The invention relates to nanoparticles which are composed by a hollow silica nanostructure in which negative charged gold seeds are embedded in its central cavity by positive-charged polymer. In this system, every component is synergistically associated to the other, resulting in a complex object able to reach the target in the organism, to produce the theranostics action, and finally to be biodegraded and cleared out. The nanoparticles can be used in in vivo analysis of the systems with theranostics features (photoacoustic and x-ray enhancement).

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Steerable probe based on interlaced continuum devices based on follow the leader approach

The invention relates to a continuously steerable robotic probe composed of two interlaced continuum robots that advance over a chosen smooth trajectory by a follow-the-leader strategy. Physical track-building is achieved by an alternating method: each continuum robot is alternatively actively stiffened to guide the other, and loosened to be guided on the other.

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GBSA-Gd NPs for MRI in cancer diagnostic applications

The present invention provides a method to produce controllable sizes of protein nanoparticles (NPs), including gadolinium, in a sub-100 nm size range. The method uses a desolvation process by which bovin serum albumin (BSA) molecules self-assembly into NPs, during the addition of desolvation agents (i.e., ethanol or acetone). The method includes preparing of an aqueous solution, which contains amounts of protein molecules in a precise ratio with gadolinium molecules. After the desolvation and cross-linking processes, hybrid NPs containing both BSA and Gd3+ ions are formed in a controlled size range of 35-200 nm. The NPs are made to entrap magnetic resonance contrast agents (e.g., gadolinium chloride, GdCl3 or Gd-DTPA) and can be used as novel drug formulation for magnetic resonance imaging (MRI). Moreover, the formulation can be applied for enhancing the MRI signal of tumours in mammals.

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Macrophage-based targeted delivery system of ferritin bound compounds

The invention relates to a macrophage-based targeted delivery system of ferritin bound active ingredients that is used for directing the desired active ingredients directly to the tumor mass or other hypoxic areas within the patient’s body, i.e. the area targeted by macrophages. This system is also useful for delivery of a contrast agent to enable imaging of macrophage targeted area or for delivery of active ingredients (drugs or prodrugs) to the tumor mass or hypoxic area for treatment purposes.

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