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Enrica Petrini

Researcher

Research Line

Synaptic Plasticity of Inhibitory Networks

Center

IIT Central Research Labs Genova

Contacts

Neuroscience and Brain Technologies Deptvia Morego 30
+39 010 2896 726
Contact Me

About

Enrica Maria Petrini graduated in Medical Biotechnology at the University of Bologna in 2000 studying gas-1 and ceramidase in cell cycle regulation. In 2000 she underwent a postdegree training on the yeast two hybrid system to identify interactors of the human homologue of B99 at Laboratorio Nazionale C.I.B. (Consorzio Interuniversitario Biotecnologie) in Trieste under the supervision of Prof. Schneider. From 2001 to 2004 she accomplished her PhD studies at the Department of Biophisics - Neurobiology at SISSA (International School for Advanced Studies) under the supervision of Prof. Cherubini studying the influence of GABAA receptor clustering on GABAergic synaptic and tonic inhibition in cultured hippocampal neurons. During the same period she also focused on the biophysics of GABAAR gating and on presynaptic sources of quantal size variability at GABAergic synapses. In 2004 she moved to France for a post-doctoral training in fluorescence imaging and single particle tracking in Prof. Choquet’s lab in Bordeaux to study the influence of AMPAR endocytosis on receptor surface mobility. In 2006 Dr. Petrini obtained a Marie Curie fellowship (IEF) to investigate the contribution of AMPA receptorlateral diffusion and receptor recycling during long term potentiation at glutamatergic synapses. Currently she holds a Research Scientist position at Italian Institute of Technology (IIT) in Genoa, in Dr. Barberis’ lab, studying the role of receptor dynamics in the regulation of synaptic function and in the computational properties of the neuron.

Dr. Petrini has a solid background in neurophysiology with a keen interest on the nano-scaled organization and dynamics of synaptic proteins, on membrane trafficking and on the impact of receptor dynamics on synaptic physiology. Over the years she has combined her wide technical experience in leading-edge imaging techniques (e.g. Single Particle Tracking, high-resolution live cell imaging, multicolor fluorescence recovery after photobleaching, confocal and multiphoton microscopy), electrophysiology, immunocyto/histochemistry, cellular and molecular biology for a high resolution investigation of the molecular organization and function of the synapse.

Projects

 Molecular determinants of inhibitory synaptic plasticity

This project aims at understanding the molecular modifications responsible for potentiation at GABAergic synapses (iLTP) and the contribution of GABAAR lateral mobility in neuronal computation. With multidisciplinary approaches combining electrophysiology, single particle tracking, super resolution microscopy, biochemistry and immunochemistry we are focusing on:

  • The modulation of surface GABAAR lateral diffusion for the rapid adjustment of synaptic receptor number during iLTP
  • The reorganization of the postsysnaptic scaffold for iLTP expression
  • Post-translational modifications responsible for iLTP

 

Inhibitory synaptic plasticity in the NL3-R451C mouse model of autism spectrum disorders

Autistic Spectrum Disorder (ASD) has been associated to genetic alterations of proteins that are crucial for the synaptic function such as Neuroligin 3 (NL3). This projects focuses on the R451C mutation on NL3 -found in autistic siblings- as a molecular determinant of ASD. We aim at assessing how this mutation affects the structure and function of inhibitory synapses and alters inhibitory synaptic plasticity in a characterized mouse model of ASD, the NL3R451C knock-in (KI).

 

Influence of receptor endocytosis on inhibitory synaptic function through the modulation of GABAA receptors availability and stabilization at GABAergic synapses

The availability of neurotransmitter receptors at synapses is a key determinant of synaptic transmission known to depend on receptor intracellular trafficking and on surface receptor lateral mobility. In this context the location and dynamics of GABAA receptor exocytosis and endocytosis can significantly shape synaptic inhibition. This project explores how posttranslational modifications responsible for the regulation of GABAA receptor internalization influence inhibitory synaptic responses by tuning receptor surface dynamics and receptor-scaffold interactions. Additionally, we investigate how those processes are scaled when promoted GABAA receptor exocytosis sustains the expression of inhibitory long-term potentiation (iLTP) of inhibitory synapses.

 

Selected Publications

 

OrlandoM*, Ravasenga T*,Petrini EM*, Falqui A, Marotta R, Barberis A.
Correlating fluorescence and high-resolution scanning electron microscopy (HRSEM) for the study of GABAA receptor clustering induced by inhibitory synaptic plasticity
Scientific Reports (2017); doi: 10.1038/s41598-017-14210-5.
* equal contribution
 
de Luca E*, Ravasenga T*, Petrini EM*, Polenghi A, Nieus T, Guazzi S, Barberis A.
Intersynaptic lateral diffusion of GABAA receptors shapes inhibitory synaptic currents
Neuron (2017); doi: 10.1016/j.neuron.2017.06.022.
* equal contribution
 
Pennacchietti F, Vascon S, Nieus T, Rosillo C, Das S, Tyagarajan SK, Diaspro A, Del Bue A, Petrini EM, Barberis A, Cella Zanacchi F.
Nanoscale molecular reorganization of the inhibitory postsynaptic density is a determinant of GABAergic synaptic potentiation
Journal of Neuroscience (2017); doi: 10.1523/JNEUROSCI.0514-16.2016
 
Petrini E.M., and Barberis A.
Diffusion dynamics of synaptic molecules during inhibitory postsynaptic plasticity. 
Frontiers in Cellular Neuroscience (2014);  doi: 10.3389/fncel.2014.00300

Petrini E.M.
, Ravasenga T., Hausrat T., Iurilli G., Olcese U., Racine V., Sibarita J.B., Jacob T.C., Moss S., Benfenati F., Medini P., Kneussel M., and Barberis A.
Synaptic recruitment of gephyrin regulates surface GABAA receptors dynamics for the expression of inhibitory LTP.
Nature Communications (2014); June 4, 3921 (5) doi:10.1038/ncomms4921

Petrini E.M., Nieus T., Ravasenga T., Succol F., Guazzi S., Benfenati F., Barberis A.
Influence of GABAAR Monoliganded States on GABAergic Responses.
Journal of Neuroscience (2011) 31(5):1752–1761

Petrini E.M., Lu J.; Cognet L., Lounis B., Ehlers M.D., Choquet D.
Endocytic Trafficking and Recycling Maintain a Pool of Mobile Surface AMPA Receptors Required for Synaptic Potentiation. 
Neuron (2009) 63, 92–105

Petrini E.M., Marchionni I., Zacchi P., W. Sieghart, Cherubini E.
Clustering of extrasynaptic GABAA receptors modulates tonic inhibition in cultured hippocampal neurons. 
Journal of Biological Chemistry(2004); 279(44):45833-43

Petrini E.M., Zacchi P., Barberis A., Morzyzmas J.W., Cherubini E.
Declusterization of GABAAR affects the kinetic properties of GABAergic currents in cultured hippocampal neurons. 
Journal of Biological Chemistry(2003); 278(18):16271-16279

Awards

2004          Travel Fellowship Award, FENS Forum, Lisboa, Portugal

2006          Marie Curie Fellowship (FP6), Intra European Fellowship

2008          Travel Fellowship Award, MN2008 Meeting, Milan, Italy

2008          Molecular Mechanisms in Neuroscience, Milan, Italy, Best Poster

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