His main research activity aims at the biophysical study of biological molecules and macromolecules both in situ and in vitro utilizing almost not perturbative instrumentation and nanostructured model systems. So far, his research deals with the design, realization and utilization of biophysical instrumentation as conventional, confocal and multi-photon fluorescence microscopy, SHG imaging microscopy, FLIM, single molecule imaging, STED nanoscopy. In particular, he developed an original setup for SW 2PE-STED (single wavelength two-photon excitation stimulated emission depletion) super-resolution microscopy and another for SHG (second harmonic generation) combined with 2PEF (2-photon excitation fluorescence) microscopy. He recently applied STED nanoscopy technique to the quantitative study of single molecule diffusion in living cells. Moreover, he is in charge of the scientific management of the Nikon Imaging Center and he is responsible for the partnership with the R&D of Nikon Instruments Japan.
Phone
+39 010 2897 613
Research center
CHT@Erzelli
Biografia
Top Publications
2021
Storti B., Quaranta P., Di Primio C., Clementi N., Mancini N., Criscuolo E., Spezia P.G., Carnicelli V., Lottini G., Paolini E., Freer G., Lai M., Costa M., Beltram F., Diaspro A., Pistello M., Zucchi R., Bianchini P., Signore G., Bizzarri R.
A spatial multi-scale fluorescence microscopy toolbox discloses entry checkpoints of SARS-CoV-2 variants in Vero E6 cells
Computational and Structural Biotechnology Journal, vol. 19, pp. 6140-6156
Article
Journal
2021
Jadavi S., Bianchini P., Cavalleri O., Dante S., Canale C., Diaspro A.
Correlative nanoscopy: A multimodal approach to molecular resolution
Microscopy Research and Technique, vol. 84, (no. 10), pp. 2472-2482
2020
Bianchini P., Saggau P., Saggau P., Diaspro A., Diaspro A.
An inertia-free beam scanning device for single-wavelength 2PE-STED nanoscopy
Journal of Physics D: Applied Physics, vol. 53, (no. 32)
2020
Deguchi T., Bianchini P., Palazzolo G., Oneto M., Diaspro A., Duocastella M.
Volumetric Lissajous confocal microscopy with tunable spatiotemporal resolution
Biomedical Optics Express, vol. 11, (no. 11), pp. 6293-6310
2019
Cosentino M., Canale C., Bianchini P., Diaspro A.
AFM-STED correlative nanoscopy reveals a dark side in fluorescence microscopy imaging
Science advances, vol. 5, (no. 6)
All Publications
2025
Mariangeli M., Moreno A., Delcanale P., Abbruzzetti S., Diaspro A., Viappiani C., Bianchini P.
AFM and fluorescence microscopy for the study of viral particles
Il Nuovo cimento della Societá italiana di fisica. C, vol. 48, (no. 4)
2025
Mussini A., Mariangeli M., Delcanale P., Abbruzzetti S., Bianchini P., Diaspro A., Lelli D., Moreno A., Viappiani C.
Angiotensin Converting Enzyme 2 is a molecular decoy for targeted photodynamic SARS-CoV-2 inactivation
International Journal of Biological Macromolecules, vol. 320
2025
Incardona N., Oneto M., Castello M., Bianchini P., Diaspro A.
Combined fluorescence-Mueller-matrix microscopy for the characterization of chiral structures organization
Proc. SPIE - Optical Methods for Inspection, Characterization, and Imaging of Biomaterials, vol. 13571
2025
Incardona N., Bianchini P., Diaspro A.
Darkfield Mueller matrix microscope allows the observation of single cells with high contrast
Optics Letters, vol. 50, (no. 21), pp. 6750-6753
2025
Auditto S., Carzino R., Summa M., Oneto M., Scotto M., Paknezhad N., Lauciello S., Bianchini P., Bertorelli R., Athanassiou A.
Electrodeposited Functional Coatings for On-Demand Electro-Responsive Drug Release and Cell Shape Transformation
https://chemrxiv.org/
Article
E-print Archive
Awards and Achievements
2025
Bianchini P.
Vice-Chair for Data Management of the International Advisory Board at Czech Bioimaging research infrastructure
2013
Bianchini P.
TR35-Young Innovators Italia
Editorships
2024
Bianchini P.
IEEE Photonics Journal
2020-2020
Bianchini P.
Frontiers in Physics
2018-2023
Bianchini P.
European Biophysics Journal
2018-2025
Bianchini P.
Microscopy Research and Technique
2014
Bianchini P.
Frontiers in Bioengineering and Biotechnology, Nanobiotechnology
Organized Events
2025
Diaspro A., Bianchini P.
10th NIC@IIT Advanced Microscopy practical workshop
2025
Diaspro A., Bianchini P.
International Day of Light
2025
Diaspro A., Bianchini P.
Retreat Nanoscopy 2025
2024
Diaspro A., Bianchini P.
9th NIC@IIT and MOSBRI Advanced Microscopy practical workshop
2024
Diaspro A., Bianchini P., Engelbrecht L.
Correlative Multimodal Imaging Workshop