BrightEyes

BrightEyes
ERC Consolidator Grant 2019-2024

Multi-Parameter Live-Cell Observation of Biomolecular Processes with Single-Photon Detector Array

Understanding how biomolecules behave - in a structural and dynamics sense - is the holy grail of cell biology research. The cell is a crowed and ever-changing environment where biomolecules jostle around, interact, concentrate, change in structure and organize in a hierarchical way to carry out all the process that regulate life. Deciphering the bimolecular processes underlying the physiology of a cell is fundamental to understand human health, ageing, and diseases. The overall objective of the BrightEyes project is to develop a set of innovative and non-invasive imaging and spectroscopy tools able to observe a single-biomolecule at work in a living multi-cellular system. Specifically, by exploring novel single-photon detectors arrays, the BrightEyes project will implement an optical system able to continuously (i) track in real-time a biomolecule of interest; (ii) measure its nano-environment and its structural changes; (iii) observe its interactions with other biomolecules; (iv) visualize its sub-cellular micro-environment with nanometre resolution.