The research in my lab aims to engineer and generate novel bioinspired protein scaffolds that can self-assemble upon stimulation into desired well-ordered and stable multicomponent nano-biostructures. This process is strongly driven by specific technological needs. Engineering protein molecules that self-assemble into complex bioarchitectures is an innovative goal of nanobiotechnology. Applications can range from design of bioactive 3D nanobiomaterials to cascade biocatalysis in sustainable technology, from bioelectronics to biomolecular medicine.
Our research interests also focus on the structure | function | interactions of innovative membrane protein targets, relevant for metabolic disorders and inflammation, and the discovery of privileged chemical structures as leads to novel drugs.
Primary research tools include approaches of structural biophysics and biotechnology. Among these, we use maily protein design & engineering, x-ray diffraction & electron crystallography, single particle cryo-electron microscopy, mass spectrometry, surface plasmon resonance, microcalorimetry, supported by computational methods. These methods allow us to describe the details of biomolecular structures, and the thermodynamics and kinetics of protein interactions.
Gianpiero Garau (P.I.)
Eleonora Margheritis (postdoc)
Valentina De Lorenzi (postdoc),
Sara Chiarugi (PhD fellow)
Elisa Martino (PhD fellow)
Aurora Russo (student).
> BioStructure Design
With this project we aim to generate novel bioinspired protein scaffolds that can self-assemble upon stimulation into desired well-ordered and stable multicomponent nanobiostructures for specific technological needs.
This project is supported by grant funding from the CANON FOUNDATION IN EUROPE.
> NAPE-PLD Interactions in Metabolic Diseases
The membrane-associated enzyme NAPE-PLD generates bioactive lipid amides that play important roles in stress and pain response, appetite and lifespan. Our studies have shown the molecular mechanism of their biogenesis and unveiled that the natural bile acids drive it. This discovery brings together bile acid physiology and lipid amide signaling, linking major players in lipid homeostasis with major players in lipid signaling. Small-molecule modulators of NAPE-PLD can have application in several metabolic and inflammatory disorders.
This project is carried out with financial support from the Italian Ministery of Foreign Affairs and Internation Cooperation (MAECI) [ http://www.esteri.it/mae/en ] ( Italy-Israel Joint Innovation Program for Industrial, Scientific and Technological Cooperation in R&D, Project SBD2-CNI-BGU ), and the European Commission (FP7 Project N. 268385) [ http://cordis.europa.eu/result/rcn/141649_en.html ].
Longevity and healthy ageing are affected by our diet and lifestyle. EU-funded researchers have associated certain organic compounds called fatty acid ethanolamides (FAEs) with obesity and ageing
BOOK: Beta-Lactamases. Ed. Jean-Marie Frère, Nova Publishers 2011.
X-ray structures and mechanisms of metallo-beta-lactamases.
Gianpiero Garau, Isabel Garcia-Saez, Laurent Chantalat, Andrea Carfi, Otto Dideberg.
Chapter 3, pp. 41-77.
HIGHLIGHTS: Synchrotron ELETTRA Research 2005-2006.
Wuerges J, Garau G, Geremia S, Randaccio L.
Crystal structure of human and bovine Vitamin B12-transport protein Trascobalamin.
Section Structural Biology, pp. 71-73.
Publications, Citations, H-index: