Agnieszka Pierzynska-Mach studied biophysics at the Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University (Cracow, Poland) and graduated in 2011. Her master thesis was focused on intracellular transport and usage of low molecular weight dyes for detection lysosomes in living cells. In years 2012-2015 she completed interdisciplinary studies for PhD students "Society - Technology - Environment" (a project realized within the Human Capital Operational Programme, Jagiellonian University, Cracow, Poland) as well as postgraduate studies "Project management" (Cracow University of Economics, Poland) and received postgraduate diploma. In 2011-2015 she attended to a 4 year PhD programme in Biochemistry, Biophysics and Molecular Biology at the Faculty of Biochemistry, Biophysics and Biotechnology. She received PhD Degree in the field of Biophysics (with distinction) in 2017. Her PhD thesis (under the supervision of Prof. Jerzy Dobrucki) concerned subnuclear localization and effectiveness of UVC-induced DNA repair in human secondary fibroblasts investigated using confocal and super-resolution microscopy.
Since December 2017 she works at the Nanophysics Department as a Post Doc. Her current research activity is focused on the use of advanced microscopy techniques and super-resolution techniques based on localization of single molecules to define the structure and organization of chromatin within the cell nucleus. She is interested in a quantitative study of the nuclear distribution of chromatin and nuclear proteins playing an architectural role, using single molecule localization techniques. She conducts the nanoscale study by using cluster analysis algorithms and DNA origami for data calibration.
“Measurement of number of repair factors molecules gathered in region of DNA repair”; Project Leader – funding received from program KNOW (Leading National Scientific Centre)
V.2008 – VII.2011
“Quantitative registration, analysis and interpretation of microscopic biological data”; N N301 465843 and Molecular Biotechnology for Health (POIG.02.01.00-12-064/08); Research Team Member
III – VIII.2010
"High resolution visualization of living cells by a new microscopy method CW STED" awarded as the Student Research Project, funding received from the Dean of the Faculty of Biochemistry, Biophysics and Biotechnology prof. Wojciech Froncisz, the grant of the Ministry of Science and Higher Education for Molecular Biotechnology health (POIG.02.01.00-12-064/08)
A. Pierzyńska-Mach, A. Szczurek, F. Cella Zanacchi, F. Pennacchietti, J. Drukała, A. Diaspro, C. Cremer, Z. Darzynkiewicz, J. W. Dobrucki, “Subnuclear localization, rates and effectiveness of UVC-induced unscheduled DNA synthesis visualized by fluorescence widefield, confocal and super-resolution microscopy”; Cell Cycle, 15(8):1156-67
A. Pierzyńska-Mach, P.A. Janowski, J. Dobrucki - Evaluation of acridine orange, LysoTracker Red, and quinacrine as fluorescent probes for long-term tracking of acidic vesicles”; Cytometry A, 85(8): 729-37
J. Kwiatek, A. Pierzyńska-Mach, P. Rybak, M. Zawrotniak, J. Dobrucki - „Visualization of living cells by light microscopy STED CW” in peer-reviewed conference proceedings – I National Conference of Biophysics Students; ISBN 978-83-61026-50-1, AT Group Sp z o.o. ul. Władysława Łokietka 48 31-334 Kraków
A. Pierzyńska-Mach, J. Dobrucki – „Imaging of acidic vesicles in living human fibroblast cells with low molecular weight fluorescent dyes: acridine orange and quinacrine”
Article in peer-reviewed conference proceedings Internet Promotion of Science realized within funds of European Social Fund under the Human Capital Operational Programme – Activity 4.2. Development of qualification of R&D system and increase of awareness of the role of science in economic development