Bio
Alberto Diaspro was born in Genoa, Italy, on April 7, 1959. He received is doctoral degree in electronic engineering from the University of Genoa, Italy, in 1983. He is University Professor in the Applied Physics Area. He is Head of the Nanophysics Unit of the Italian Institute of Technology, www.iit.it. AD is member of the University advisory Commitee for PhD Courses in Biotechnology (University of Genoa) and Medical Nanotechnology (University of Milan). AD has collaborations with IFOM - FIRC Oncology Institute, Milan, the Institute of Biophysics of the National Research Council (CNR) and NBT-IIT, Italian Institute of Technology. AD founded LAMBS (Laboratory for Advanced Microscopy, Bioimaging and Spectroscopy, www.lambs.it). AD is author of more than 170 international ISI publications. He is the President of OWLS (Optics With Life Sciences) and ICO (International Commission of Optics) representative. AD was the President Elect of EBSA (European Biophysical Societies\\\' Association, www.ebsa.org) and founder of the Biophysical Society Nanoscale Biophysics subgroup, www.biophyscs.org. AD is active memeber of OSA, SPIE, SIF (Italian Physical Society, www.sif.it). AD has been invited as speaker to more than 200 international conferences. Recently he was the co-organizer of a World Conference on Microscopy, FOM2003, SIF 2008 (Italian Physical Society) and of EBSA 2009 (www.ebsa2009.org). He was visiting scientist at Drexel University, Philadelphia, USA, Universidad Autonoma de Madrid, Madrid, Spain, Academy of Sciences of Czech Rpeublic, Polytechnich University of Bucharest, Romania, University of Illinois, Urbana-Champaign, USA.
Microscopy skills
Confocal and Multiphoton Microscopy, i.e., FRAP, FRET, SHG, lifetime imaging, spectral fingerprint, single molecule detection, colocalization, 3D/4D.
Live imaging down to molecular resolution, single molecule/particle tracking with 5-10 nm accuray, 100-40 nm resolution, single molecule sensitivity, Nanoscopy - FCS, TIRF, STED/PALM, IML-SPIM (Individual Molecule Localization - Selective Plane Illumination Microscopy).
4D (x, y, z, t) particle tracking, fast scanning modes - 100 fps, 512x512 pixel, photoactivatable fluo markers - paGFP, other fluo probes.
Active optical microscopy molecular 3D/4D uncaging and events follow up.
Deep imaging towards small animal live imaging, depth penetration, 0.5-1 mm.
Integration with electrophysiological data, multimodal platform for simultaneous imaging and data analysis.
Implementation of a deconvolution and image processing platform powermicroscope - friendly remote web access, correlative microscopy vs. TEM, SEM, AFM.
Diaspro experience is related with the design, realization and utilization of biophysical instrumentation as conventional and confocal microscopy, two-photon fluorescence microscopy and spectroscopy architecture, differential scanning calorimetry, scanning probe microscopy (STM, SNOM, AFM), polarized light scattering, signal and image digital processing. At Diaspro Lab was realised the first italian two-photon excitation architecture for microscopy and spectroscopy with extension to single molecule imaging. As well a nanotechnological approach for the realization of Nanobiorobot, hybrid nanostructured devices made by living cells and polylelctrolyte layers, has been recently developed. Diaspro is currently involved in the design and realization of the first italian Nanoscopy architecture.
Other Publications
Selected Most Recent publications
Single-wavelength two-photon excitation-stimulated emission depletion (SW2PE-STED) superresolution imaging. Bianchini P, Harke B, Galiani S, Vicidomini G, Diaspro A. Proc Natl Acad Sci U S A. 2012 Apr 24;109(17):6390-3.
A novel nanoscopic tool by combining AFM with STED microscopy. Harke B, Chacko JV, Haschke H, Canale C and Diaspro A. Optical Nanoscopy 2012, 1:3 (25 April 2012)
Strategies to maximize the performance of a STED microscope. Galiani S, Harke B, Vicidomini G, Lignani G, Benfenati F, Diaspro A, Bianchini P. Opt Express. 2012 Mar 26;20(7):7362-74.
Photopolymerization inhibition dynamics for sub-diffraction direct laser writing lithography. Harke B, Bianchini P, Brandi F, Diaspro A. Chemphyschem. 2012 Apr 23;13(6):1429-34.
Probing cytoskeleton organisation of neuroblastoma cells with single-cell force spectroscopy. Mescola A, Vella S, Scotto M, Gavazzo P, Canale C, Diaspro A, Pagano A, Vassalli M. J Mol Recognit. 2012 May;25(5):270-7.
Order versus Disorder: in vivo bone formation within osteoconductive scaffolds.
Scaglione S, Giannoni P, Bianchini P, Sandri M, Marotta R, Firpo G, Valbusa U, Tampieri A, Diaspro A, Bianco P, Quarto R. Nature Sci Rep. 2012;2:274.
Bioconjugated silicon quantum dots from one-step green synthesis. Intartaglia R, Barchanski A, Bagga K, Genovese A, Das G, Wagener P, Di Fabrizio E, Diaspro A, Brandi F, Barcikowski S. Nanoscale. 2012 Feb 21;4(4):1271-4.
Plasmon bleaching dynamics in colloidal gold-iron oxide nanocrystal heterodimers.
Comin A, Korobchevskaya K, George C, Diaspro A, Manna L. Nano Lett. 2012 Feb 8;12(2):921-6.
Fast scanning STED and two-photon fluorescence excitation microscopy with continuous wave beam. Bianchini P, Diaspro A. J Microsc. 2012 Mar;245(3):225-8.
Fabrication of large-area ordered and reproducible nanostructures for SERS biosensor application. Das G, Patra N, Gopalakrishnan A, Zaccaria RP, Toma A, Thorat S, Di Fabrizio E, Diaspro A, Salerno M. Analyst. 2012 Apr 21;137(8):1785-92.
Inhibiting effect of α(s1)-casein on Aβ(1-40) fibrillogenesis. Carrotta R, Canale C, Diaspro A, Trapani A, Biagio PL, Bulone D. Biochim Biophys Acta. 2012 Feb;1820(2):124-32.
Live-cell 3D super-resolution imaging in thick biological samples. Cella Zanacchi F, Lavagnino Z, Perrone Donnorso M, Del Bue A, Furia L, Faretta M, Diaspro A. Nature Methods. 2011 Oct 9;8(12):1047-9.
Magnetic force microscopy and energy loss imaging of superparamagnetic iron oxide nanoparticles. Torre B, Bertoni G, Fragouli D, Falqui A, Salerno M, Diaspro A, Cingolani R, Athanassiou A. Nature Sci Rep. 2011;1:202.
Simultaneous multilayer scanning and detection for multiphoton fluorescence microscopy. Mondal PP, Diaspro A. Nature Sci Rep. 2011;1:149.
Very large spot size effect in nanosecond laser drilling efficiency of silicon. Brandi F, Burdet N, Carzino R, Diaspro A. Opt Express. 2010 Oct 25;18(22):23488-94.
